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Real-Time PCR - General
What is the advantage of working with a probe system?
A probe system is always specific (except Amplifluor™ probes) and therefore does only detect the gene of interest. If you have a difficult to optimize PCR it will not show you any primer dimers or aspecific products.
With a probe system it is also possible to distinguish between similar sequences with small differences like SNPs or mutations.
In general, probe assays need less optimisation than SYBR® Green I assays.
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