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Real-Time PCR - General
Why do the qPCR and RT qPCR kits contain HotGoldStar, a hotstart Taq polymerase?
Aspecific products influence the results of a Real-Time PCR in a negative way leading to wrong quantitation. To avoid the amplification of aspecific products and accumulation of primer dimers during the initial heating phase of the PCR a hotstart Taq polymerase is used, which only becomes active after heating at 95 °C for 10 minutes.
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