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FAQs
 
Real-Time PCR - General Back to the categories list
What is the advantage of working with SYBR® Green I?
What is the advantage of working with a probe system?
Is a probe assay more sensitive than a SYBR® Green I assay?
Can I use a SYBR® Green I kit for a probe assay?
How should the qPCR and RT qPCR kits be stored?
What is the difference between a Core kit and a MasterMix?
What is the difference between a MasterMix and a MasterMix Plus?
Should the DNA and the qPCR MasterMix be mixed before thermocycling?
What is the difference between a one step and a two step RT qPCR reaction?
In which case is one step RT qPCR recommended?
Why is a Two step RT qPCR kit more efficient than a One step RT qPCR kit?
Why do the qPCR and RT qPCR kits contain HotGoldStar, a hotstart Taq polymerase?
Why do the Eurogentec RT qPCR kits contain EuroScript, a MmLV reverse transcriptase?
Why do qPCR kits contain dUTP (and UNG)?
Why does an initial step at 50 °C during 2 min and a second step at 95 °C during 10 min have to be performed when using UNG for carry over prevention?
Why do the One step RT qPCR MasterMixes not contain UNG?
Why do certain qPCR kits contain ROX passive reference?
Can an ABI kit with a ROX passive reference be used on an IQ i-cycler?
What should I do to use a ROX or a No ROX qPCR Core kit or MasterMix for SYBR® Green I correctly on an IQ i-cycler?
How do I avoid amplification of genomic DNA, which can be a contamination factor in my isolated RNA in RT qPCR?
Why does the protocol for "qPCR MasterMix" state: hold at 50 °C forever (at the end of the cycling program)?

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