The Plexor™ primer technology is a technique for Real-Time qPCR adapted to multiplex assays. This technology takes advantage of the highly specific interaction between two modified nucleotides. 

These two novel bases, called isoguanine (iso-dG) and 5 -methylisocytosine (iso-dC), form a unique base pair when incorporated in double-stranded DNA and pair only with each other. In Plexor™ reactions, one PCR primer is synthesized with an iso-dC residue and a fluorescent label at the 5-end. The second PCR primer is a standard unlabelled oligonucleotide. 

Eurogentec is a referenced supplier of Plexor™ labelled and unlabelled primers. Validated positive control sets containing a pair of labelled and unlabelled primers are also available from us. Please note that correct resuspension is critical for optimal use of Plexor™ primers.