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Acridine

Life Science Molecular Diagnostics GMP BioManufacturing
Acridine Build & Order
Oligos bearing the 6-chloro-2-methoxyacridine molecule at either terminus or within the sequence have the ability to intercalate efficiently into a double helix. Covalent binding of 6-chloro-2-methoxyacridine therefore increases hybrid stability by providing additional binding energy. Acridine-labelled oligos can thus be used in applications where increased stability of oligonucleotide hybrids is crucial. Adding the dye to the 3’terminus also protects the oligo from exonuclease degradation.

These oligos also possess the ability to form triplex helices, and, due to their relative hydrophobicity, to pass through membranes more easily than normal oligos.

Name Synthesis scale Recommended Purification* Required Purification* Reference EUR
5' MeO-Cl-Acridine 40 nmol RP-HPLC - MD-CN050-05004 101.00
5' MeO-Cl-Acridine 200 nmol RP-HPLC - MD-CN050-05020 158.00
5' MeO-Cl-Acridine 1000 nmol RP-HPLC - MD-CN050-05100 200.50
3' MeO-Cl-Acridine 40 nmol RP-HPLC - MD-CN050-03004 74.00
3' MeO-Cl-Acridine 200 nmol RP-HPLC - MD-CN050-03020 93.20
3' MeO-Cl-Acridine 1000 nmol RP-HPLC - MD-CN050-03100 158.00
Internal MeO-Cl-Acridine 40 nmol RP-HPLC - MD-CN050-IN004 101.00
Internal MeO-Cl-Acridine 200 nmol RP-HPLC - MD-CN050-IN020 158.00
Internal MeO-Cl-Acridine 1000 nmol RP-HPLC - MD-CN050-IN100 200.50
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                              15-39 bases: 5 Working days
                              40-80 bases: 7-8 Working days
                              > 80 bases: 10 Working days

Packaging

   Dried

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
Oligos bearing the 6-chloro-2-methoxyacridine molecule at either terminus or within the sequence have the ability to intercalate efficiently into a double helix. Covalent binding of 6-chloro-2-methoxyacridine therefore increases hybrid stability by providing additional binding energy. Acridine-labelled oligos can thus be used in applications where increased stability of oligonucleotide hybrids is crucial. Adding the dye to the 3’terminus also protects the oligo from exonuclease degradation.

These oligos also possess the ability to form triplex helices, and, due to their relative hydrophobicity, to pass through membranes more easily than normal oligos.


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   For Research Use only

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Product citations


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OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002


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RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004


SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005


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ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002


KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002


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GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N4-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004


MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004


DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004



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