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Acrylamide Build & Order

5’ acryl-labelled oligonucleotides allow the copolymerization of these oligos into polyacrylamide gels. The gel has one or more zones that contain oligos complementary to specific sequences. Passing a fragment containing a complementary sequence through such a zone results in trapping the fragment. These modified oligonucleotides find their applications in mutant detection, selecting sequences for cloning, sample preparation, concentration and diagnostics applications.

Name	Synthesis scale	Recommended Purification*	Required Purification*	Reference	EUR
5' Acrylamide (Acrydite®)	40 nmol	RP-HPLC	-	MD-CN020-05004	221.00
5' Acrylamide (Acrydite®)	200 nmol	RP-HPLC	-	MD-CN020-05020	416.00
5' Acrylamide (Acrydite®)	1000 nmol	RP-HPLC	-	MD-CN020-05100	683.00

* More info on the Purifications page

Quality Control

MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                              15-39 bases: 5 Working days
                              40-80 bases: 7-8 Working days
                              > 80 bases: 10 Working days

Packaging

Dried

Shipping conditions

Room temperature

Storage conditions

-20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Legal notices

For Research Use only

Leaflets

Oligonucleotides Catalogue

Product citations

LEGENDRE D. et al., "Engineering a regulatable enzyme for homogeneous immunoassays", Nature Biotechnology, vol. 17, n° 1, p.67-72, 1 January 1999

MOMENI P. et al., "Mutations in a new gene, encoding a zinc-finger protein, cause tricho-rhino-phalangeal syndrome type I", Nature Genetics, vol. 24, p. 71-74, 1 January 2000

PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002

OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002

GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004

RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004

SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005

VAN DER STEEGE G. et al., "Persistent failures in gene repair", Nature Biotechnology, n° 19, p. 305-306, April 2001

ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002

KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002

VANDEN ABEELE F. et al., "Store-operated Ca²⁺ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003

GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N⁴-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004

MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004

DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004

Online ordering: The most convenient way to order your oligonucleotides, peptides and Real-Time qPCR kits is to use our web ordering system. To place orders online, simply login if you have already an online account.

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By e-mail: order@eurogentec.com