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Antigen requirements and serum yields

Research Diagnostic Therapeutic
Animal host characteristics
Host Min. antigenn quantity/injection Bleed volumes
 Comment
Antigen MW <18kDa Antigen MW >18kDa Pre-immune Small Bleed Large Bleed Final Bleed
Mouse 40 μg 15 μg 30 μg in 270 μl of PBS - - 300 μl Good to test antigenicity
Guinea Pig 50μg 30μg 1 ml 1 ml 2-3 ml 7-10 ml For small serum volumes
Rat 50μg 30μg 1 ml 1 ml 2-3 ml 7-10 ml For small serum volumes
Chicken 200μg 100μg 1 egg 1 egg 10 eggs 10-20 eggs For mammalian antigens and large quantitites of Abs. 1 egg = 50 ml of rabbit serum
Rabbit 200μg 100μg 2 ml 2 ml 20 + 2 ml 60-80 + 2 ml For most applications
Goat 400μg 200μg 2 ml 2 ml 250 + 2 ml 1000 + 2 ml For large batch volumes
Pig 400μg 200μg 2 ml 2 ml 250 + 2 ml 1000 + 2 ml For large batch volumes
Sheep 400μg 200μg 2 ml 2 ml 250 + 2 ml 1000 + 2 ml For large batch volumes
Llama 400μg 200μg 2 ml 2 ml 250 + 2 ml 1000 + 2 ml Single chain Abs and for large batch volumes
Horse 400μg 200μg 2 ml 2 ml 250 + 2 ml 1000 + 2 ml For large batch volumes
Cow 400μg 200μg 2 ml 2 ml 250 + 2 ml 1000 + 2 ml For large batch volumes

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    LIEGE Science Park
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If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.




  • Online
    You can place your order online. Login to access our web ordering interface.
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

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