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Research Diagnostic Therapeutic

Antisense molecules have been shown to inhibit very efficiently the activities of specific miRNAs. Such an inhibition is correlated to an increase of the target gene(s) activity. In several reporter-gene based experiments, the co-transfection of antisense RNAs against several miRNA induced an approximately 4-fold increase in the expression of the reporter relative to cells co-transfected with a negative control inhibitor and the various miRNA reporter plasmids.

Name	Quantity	Purification	Reference	EUR
Clear-MiR™ Plus miRNA inhibitors	5 nmol	PAGE	OR-0033-XXX*	319.00

* Unique identifier corresponding to any individual miRNA.

Quality Control

MALDI-TOF Mass Spectrometry

Delivery times

5 Working days

Packaging

Lyophilized

Shipping conditions

Room temperature

Storage conditions

-20 °C to -70 °C
Store Clear-MiR™ miRNA inhibitors as a dry pellet at -20 °C (or preferably -70 °C) in a non-frost free freezer until ready to use. Once resuspended in RNase-free buffer, store at -70 °C and avoid contact with RNases.Clear-MiR™ miRNA inhibitors should be resuspended to a convenient stock concentration (20 to 50 µM) and stored in small aliquots to avoid multiple freeze thaw cycles. When stored under these conditions and using good RNase-free technique, they typically remain stable for 6 months or more. The solution can be freeze-thawed up to 5 times. For long-term storage, Clear-MiR™ miRNA inhibitors should be dried.

Clear-MiR™ miRNA inhibitors are chemically modified antisense RNA oligonucleotides optimized to specifically target miRNA molecules in cells.
Use of Clear-MiR™ miRNA inhibitors enables miRNA functional analysis for down-regulation of miRNA activity (loss-of-function mutants). These optimized miRNA Inhibitors represents essential tools to confirm or determine miRNA function.

Key features of the Clear-MiR™ miRNA inhibitors

Sequence-specific antisense inhibition of miRNA
Resistance to nucleases
Tight regulation of miRNA cellular levels
Inhibition of miRNAs both in vitro and in vivo
Available for more than 20 species and more than 3500 miRNA sequences
Minimal cytotoxicity
Easily transfected with standard methods
Increased biological stability due to proprietary formulation

Clear-MiR™ oligos are available for all known miRNAs in invertebrates, vertebrates and plants, as registered and annotated in the miRNA Registry at The Wellcome Trust Sanger Institute. Clear-MiR™ oligos are available with different labels and can be linked to cholesterol for increase cellular uptake. On request, peptides can also be covalently linked to Clear-MiR™ oligos.

How to order your Clear-MiR™ (Plus)?

Send your order (with your P.O. number, delivery and invoice addresses) by e-mail to order@eurogentec.com mentioning :

Accession Number of the corresponding miRNA
Your choice between Clear-MiR™ miRNA inhibitor and Clear-MiR™ Plus miRNA inhibitor.
We will design and synthesize the corresponding Clear-MiR™ (Plus) miRNA inhibitor.