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Inosine Build & Order

Inosine occurs naturally in the wobble position of the anticodon of some transfer RNAs and is known to form base pairs with A, C and U during the translation process.

Name	Synthesis scale	Recommended Purification*	Required Purification*	Reference	EUR
3'-dInosine	40 nmol	RP-HPLC	-	MD-NB001-03004	37.10
3' dInosine	200 nmol	RP-HPLC	-	MD-NB001-03020	58.00
3' dInosine	1000 nmol	RP-HPLC	-	MD-NB001-03100	90.00
dInosine	40 nmol	RP-HPLC	-	MD-NB001-IN004	10.50
dInosine	200 nmol	RP-HPLC	-	MD-NB001-IN020	17.00
dInosine	1000 nmol	RP-HPLC	-	MD-NB001-IN100	34.00

* More info on the Purifications page

Quality Control

MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                             15-39 bases: 5 Working days
                             40-80 bases: 7-8 Working days
                             > 80 bases: 10 Working days

Packaging

Dried

Shipping conditions

Room temperature

Storage conditions

-20°C to –70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Inosine occurs naturally in the wobble position of the anticodon of some transfer RNAs and is known to form base pairs with A, C and U during the translation process. Several experiments have suggested that deoxyinosine might be an “inert” base, its presence in an oligonucleotide sequence seems neither to disturb DNA duplex formation nor to destabilize the duplex. Independent of sequence effects, the order of base-pairing stabilities is I-C >> I-A > I-T = I-G. Deoxyinosine containing primers have been used successfully to amplify cDNA or genomic fragments for the generation of DNA probes from a peptide sequence.

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For Research Use only

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Product citations

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PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002

OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002

GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004

RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004

SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005

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ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002

KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002

VANDEN ABEELE F. et al., "Store-operated Ca²⁺ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003

GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N⁴-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004

MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004

DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004

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