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Universal Exogenous qPCR Positive Control

Real-Time PCR assays are prone to inhibition by various substances found in many samples (clinical, soil, plant…). Carryover of reagents used for the isolation of nucleic acids can also inhibit amplification reactions. Other causes of false-negative results include target nucleic acid degradation, sample processing errors and thermocycler malfunction.
Eurogentec’s Universal Exogenous qPCR positive Control provides an accurate way to assess the integrity of all the steps in a nucleic acid amplification assay.

Name #RXN (50 µl) Reference EUR
Universal Exogenous qPCR Positive Control, YY-TAMRA 200 RT-IPCY-T02 220.00
Universal Exogenous qPCR Positive Control, YY-TAMRA 1000 RT-IPCY-T10 990.00
Universal Exogenous qPCR Positive Control, YY-BHQ-1® 200 RT-IPCY-B02 220.00
Universal Exogenous qPCR Positive Control, YY-BHQ-1® 1000 RT-IPCY-B10 990.00

Delivery times

   2 WD if ordered before Thursday

Shipping conditions

   Dry ice

Storage conditions


For long-term storage, the Universal Exogenous qPCR Positive Control should be kept in the dark, at -20 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year. 
For short-term storage, the Universal Exogenous qPCR Positive Control can be kept in the dark, at  4 °C to 6 °C for one month. 
The Universal Exogenous qPCR Positive Control should be protected from light whenever possible to avoid bleaching of the probe.

Eurogentec’s Universal Exogenous qPCR Positive Control for TaqMan® Assays is an optimised Taqman control that was designed to distinguish true target negatives from false negatives due to PCR inhibition, incorrect pipetting or cycling parameters.

  • The optimised control can be spiked into samples without compromising amplification efficiency of the target sequence.
  • The optimised control doesn't match with any sequence routinely found in a lab. 
  • The optimised control is detected using a Yakima-Yellow® (VIC® equivalent)-labelled probe and the target template is detected using a FAM-labeled probe.
  • Avoid amplification of endogenous genes

High quality result achieved in 42’ with FAST qPCR MasterMix Low ROX on an ABI Prism® 7500 FAST cycler using a ten times serial dilution of 18S cDNA. (A) Dilution series of the 18S cDNA (green lines; detected in the FAM channel) performed in multiplex with the Universal Exogenous qPCR Positive Control (yellow lines;YY-BHQ-1® version, detected in the VIC®/JOE channel). (B) Dilution series of the 18S cDNA (detected in the FAM channel) performed in singleplex, without Universal Exogenous qPCR Positive Control.
The results clearly illustrate the lack of significant impact of the Positive Control amplification on the sensitivity and efficiency of the specific target amplification.




Alternatively, the Universal Exogenous qPCR Positive Control may be used in standardised conditions as extraction yield calibrator, template quality sensor or inter-run calibrator.

  • A given quantity of control can be spiked into samples before extraction. A relative (directly comparing samples) or an absolute (using a dilution curve of the control) quantification is performed after extraction to normalize the extraction yields of the samples.
  • Quantitative results of the spiked control within the template or within a reference buffer (pure water, reference template…) are compared in order to reject templates where PCR inhibition is high (low quality).
  • Add a dilution series of the optimised control on each plate and use it to normalize PCR efficiencies between plates (also for cycler to cycler data normalization).


The Universal Exogenous qPCR Positive Control kits contain a tube of 10X EGT IPC mix (blend of IPC primers and IPC probe) and a tube of 50X EGT IPC DNA.

Leaflets

TDS

MSDS

Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us
  • By e-mail: order@eurogentec.com

  • By fax: +32 4 264 07 88

  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM
If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

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