A single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions from the ends of DNA and RNA molecules, leaving blunt, ligatable ends.

Name	Quantity	Reference	EUR
Mung bean nuclease	2000 U	ME-0200-10	147.30

Delivery times

   2 working days

Shipping conditions

   -20°C

Storage conditions

   -20°C

Source
Mung bean sprouts

Applications 
Mung bean nuclease has a stringent single-stranded specificity for DNA or RNA and produces 5’-phosphoryl oligo- and mononucleotides. This enzyme is ideal for transcript mapping, removal of single-stranded regions in DNA hybrids or single-stranded overhangs produced by the restriction enzymes.

Quality Control
Tested for the absence of endo- and exonucleases.

Unit definition
The amount of enzyme that will release 1 µg of acid-soluble nucleotides in one minute from heat-denatured calf thymus DNA at 37 °C.

Notes
Mung bean nuclease requires Zn2+. The addition of EDTA or SDS causes irreversible inactivation. Do not use at pH below 4.6. Mung bean nuclease should not be used at low salt concentration.

Concentration
50 U/µl