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qPCR MasterMix Plus for SYBR® Green I w/ fluorescein

Research Diagnostic Therapeutic
qPCR MasterMix Plus ... Order
When using a SYBR® Green I kit on an iCycler iQ®, iQ5 or My iQ®, fluorescein is needed to normalize the data correctly. This is why Eurogentec has developed a SYBR® Green I kit that is already containing fluorescein in a ready-to-use MasterMix. Alternatively a separate tube of fluorescein is available in case you would like to use fluorescein in combination with any of our No ROX SYBR® Green I kits.

The qPCR MasterMix Plus for SYBR® Green I w/ fluorescein contains enough reagents for up to 300 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on iCycler iQ®, My iQ®, iQ5.


Name Quantity Reference EUR
qPCR MasterMix Plus for SYBR® Green I w/ fluorescein 300 RT-SN2X-03+NRFL 469.00
50 µl reactions

Delivery times

   2 WD if ordered before Thursday

Shipping conditions

   Dry ice

Storage conditions


For long term storage the qPCR MasterMix Plus for SYBR® Green I w/ fluorescein should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 2 years.
For short term storage the qPCR MasterMix Plus for SYBR® Green I w/ fluorescein can be stored at 4 °C to 6 °C for 1 month.
The qPCR MasterMix Plus for SYBR® Green I w/ fluorescein reaction buffer should be protected from light whenever possible.

When using a SYBR® Green I kit on an iCycler iQ®, iQ5 or My iQ®, fluorescein is needed to normalize the data correctly. This is why Eurogentec has developed a SYBR® Green I kit that is already containing fluorescein in a ready-to-use MasterMix. Alternatively a separate tube of fluorescein is available in case you would like to use fluorescein in combination of any of our No ROX SYBR® Green I kits. Our qPCR MasterMix Plus for SYBR® Green I w/ fluorescein will allow you to achieve reliable, consistent and accurate Real-Time qPCR results for SYBR® Green detection. The qPCR MasterMix Plus for SYBR® Green I w/ fluorescein contains enough reagents for up to 300 - 50 µl reactions using the hotstart enzyme, HotGoldStar and has been validated on iCycler iQ®, My iQ®, iQ5. The 2x buffer contains dNTP/dUTP, HotGoldStar, UNG, MgCl2, SYBR® Green I, fluorescein and stabilizers. An extra tube of 50 mM MgCl2 is provided in the kit to optimize MgCl2 concentration if necessary.

Legal notices


FOR RESEARCH USE ONLY - NOTICE TO PURCHASER: LIMITED LICENSE - Licensed dsDNA-Binding Dye Research Kits - Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Cyclic-substituted unsymmetrical cyanine dyes are covered by U.S. Patents 5,436,134 and 5,658,751 and licensed to Eurogentec S.A by Molecular Probes, Inc in the direct research field.

Cyclic-substituted unsymmetrical cyanine dyes are covered by U.S. Patents 5,436,134 and 5,658,751 and licensed to Eurogentec S.A by Molecular Probes, Inc in the direct research field.

Use of UDG employs U.S. Patents 5,035,996, 5,945,313, 5,683,896 and their foreign counterparts licensed to Eurogentec, S.A. from Invitrogen Corporation.

Leaflets

TDS

MSDS

Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

Product citations


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WANG S. et al., "Histone deacetylase 1 represses the small GTPase RhoB expression in human nonsmall lung carcinoma cell line", Oncogene, n° 22, p. 6204-6213, 2003


STIEWE T. et al., "Quantitative TP73 Transcript Analysis in Hepatocellular Carcinomas", Clinical Cancer Research, Vol. 10, 626-633, January 2004


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EBNER K. et al., "Molecular Detection and Quantitative Analysis of the Entire Spectrum of Human Adenoviruses by a Two-Reaction Real-Time PCR Assay", Journal of Clinical Microbiology, vol. 43, n° 7, p. 3049-3053, July 2005


Ishibashi Y. et al., "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line", Glycoconjugate Journal, 22(1-2):53-62, February 2005


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