Microarray Microplate Hardware
The Microarray Microplate Hardware contains 96 separate wells for microarrays. The glass plate comes with your favorite surface chemistry and is detachable from the rest of the chamber for superior spotting and scanning flexibility. Each subarray measures 7.7mm x 7.7mm, providing maximum printable real estate as well as conforming to robotic liquid handling platforms.
Figure . Microplate Microarray hardware pictured from top to bottom. (1) self sealing mat, that opens and seals itself for easy pipetting (2) 96 well plate configuration top with hand operation screws that go into the base. (3) Gasket that prohibits microarray "wells" from cross contaminating (4) Glass with hydrophobic grid for easy orientation (5) Base of hardware with 96 well plate footprint.
The MicroArrays are manufactured by printing the DNA probes, suspended in optimized spotting buffer, to the glass slides showing high coupling efficiency. This spotting chemistry provides the highest hybridization intensities, which are a consequence of a better binding of the probe to the slide and their accessibility to the cDNA targets.
The MicroArrays are printed with the VersArray® ChipWriter Pro System (Bio-Rad), a high precision dispensing robot. This robot is able to produce 1.25 µm resolution (x,y axis), 0.25 µm resolution (z axis), with 3 µm repeatability and 10 µm global accuracy using zero deflection, special proprietary alignment techniques and sub-micron level resolution instrumentation.
In order to generate high quality spots, the printing procedure is performed under a double-glass insulated cabinet with positive air pressure, HEPA filter and humidifier. This system allows to avoid dust, to control the spotting quality (morphology, spot diameter and uniformity) and DNA sample evaporation.
| Name |
Quantity |
Reference |
EUR |
|
| Spotting on 96 well plates |
1 |
AR-WGrid-01 |
Upon request |
|