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Spotting on glass slides

  

The MicroArrays are manufactured by printing the DNA probes, suspended in optimized spotting buffer, to the glass slides showing high coupling efficiency. This spotting chemistry provides the highest hybridization intensities, which are a consequence of a better binding of the probe to the slide and their accessibility to the cDNA targets.
 










The MicroArrays are printed with the VersArray® ChipWriter Pro System (Bio-Rad), a high precision dispensing robot. This robot is able to produce 1.25 µm resolution (x,y axis), 0.25 µm resolution
(z axis), with 3 µm repeatability and 10 µm global accuracy using zero deflection, special proprietary alignment techniques and sub-micron level resolution instrumentation. 

In order to generate high quality spots, the printing procedure is performed under a double-glass insulated cabinet with positive air pressure, HEPA filter and humidifier. This system allows to avoid dust, to control the spotting quality (morphology, spot diameter and uniformity) and DNA sample evaporation. 

Name Quantity Reference EUR
Spotting PCR on glass slides 1 AR-PGRID-GS Upon request
Spotting oligonucleotides on glass slides 1 AR-OGRID-GS Upon request


References::
Ruby et al : J Virol. 2006 September; 80(18) 92079216) Nucleus-encoded plastid sigma factor SIG3 transcribes specifically the psbN gene in plastids
Suleau et al: (FEMS Yeast Research 5 (6-7), 595–604: Intraspecific gene expression variability in the yeast Kluyveromyces lactis revealed by micro-array analysis
  • For specific quote and more information, please contact Dr Driss Talibi:

    dr.talibi@eurogentec.com
    Tel.: +32 4 372 74 39
    Mobile: +32 478 366 619
    Fax: +32 4 372 75 00

  • Online
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    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

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