Create your own peptide library
Using a new modification of standard Fmoc chemistry for peptide synthesis, Eurogentec offers the synthesis of custom peptide libraries in 96-well format for high-throughput screening purposes.
Peptide libraries include - 200 - 500 µg of each peptide
- 96-well format
- Unbound, free crude peptides
- Purification by selective precipitation (SePop)
- Lengths between 5-18 amino acids
- Amino N-terminus
- CONH2 C-terminus
- MALDI-TOF QC on 10 % of peptides
Applications - Epitope mapping
- Alanine walking
- Single amino acid mutation screening
- Protein-protein interaction studies
- Kinase motif discovery
- Protease motif discovery
Epitope Mapping Epitopes recognized by antibodies are commonly 6 amino acids in length. By generating overlapping 15 mer peptides, each shifted by 4 amino acids, one can unequivocally determine which amino acids make up the epitope. Schematically the approach looks as follows based on the following simplified “protein” example:
...KNCSHIQPWETDCLSCLPERQDEYDPKGPKVSDG
1.KNCSHIQPWETDCLS
2........SHIQPWETDCLSCLP
3.............QPWETDCLSCLPERQ
4.....................ETDCLSCLPERQDEY
5...........................CLSCLPERQDEYDPK
6..................................CLPERQDEYDPKGPK
7........................................ERQDEYDPKGPKVSD
8...............................................DEYDPKGPKVSDG
9......................................................DPKGPKVSDG
10...........................................................GPKVSDG
T-Cell Stimulation Libraries can also be generated to see which particular peptide from an antigenic protein is responsible for a T-cell response. The design process is similar to the epitope mapping example above.
Mutation Studies using Alanine Walking By screening peptides with systematic replacement of each amino acid with alanine, one can determine to which extent a particular amino acid is required and sufficient for an interaction.
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