The MESA BLUE qPCR MasterMix Plus for SYBR^® Assay No ROX has been specially optimized for the following thermocyclers :

• LC480
• Mx3000P®, Mx3005®, Mx4000® *
• iCycler iQ®, iQ5 and My-iQ® **
• DNA Engine Opticon® 1 & 2, Chromo 4, Mini Opticon®
• RotorGene 2000, 3000, 6000
• Quantica®
• Smartcycler®

In combination with Eurogentec primers, the MESA BLUE MasterMix produces highly specific and sensitive results. 

* On the Mx3000P^®, Mx3005^® and Mx4000^®, both MasterMixes with and without ROX passive reference can be used, depending on your needs. If you want to perform a ROX normalisation on your results, we advise you to use our MESA BLUE MasterMix Plus Low ROX for SYBR^® Green Assay which has been validated on these thermocyclers.
MESA BLUE MasterMixes Plus Low Row

** When using a SYBR® Green I kit on an iCycler iQ®, iQ5 or My iQ®, fluorescein is needed to normalize the data correctly. This is the reason why Eurogentec has developed a ready-to-use MESA BLUE MasterMix containing pre-mixed fluorescein.