For many years, studies on the regulation of gene expression have focused on the control of transcription, thinking that once the gene was expressed as mRNA it would be translated and when its time was up it would simply be degraded in the cytoplasm.
However the discovery in 1998, of RNA interference (RNAi) by Andrew Fire and Craig Mellow has changed this point of view.
RNA interference is a mechanism of gene silencing at the mRNA level. This phenomenon is triggered by small interfering (si)RNAs and micro (mi) RNAs. These regulatory molecules belong to the growing class of small non-coding RNAs. siRNAs and miRNAs are capable of inhibiting gene expression by either directing the degradation of homologous mRNA targets or inducing the repression of translation of mRNA targets which have incomplete complementarity.
Features
Length 21 bases to 27 bases
Backbone RNA, LNA®, 2’O-Me RNA and all linkages
Synthesis scale 10 nmol • 40 nmol • 200 nmol • 1000 nmol *
Modifications 5’: Phosphate, FAM, Cy®3, Cy5®, HEX, TET...
3’: DABCYL, TEG-Cholesteryl, TAMRA...
Purifications SePOP, HPLC or PAGE
Quality Control MALDI-TOF MS
Format & Packaging Dried in 2 ml tube by default
Documentation Technical Data Sheet
siRNA Design Free and guaranteed
*Larger synthesis scales are available on request
