Labeling-detection

5-FAM-X, SE [6-(Fluorescein-5-carboxamido)hexanoic acid, SE] - 5 mg

102,00
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  • Cat.Number : AS-81009
  • Availability :
    In production
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Although FITC reagents have been more often used to prepare fluorescent bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents generate carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions, give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
5-FAM is a single isomer. It is one of the most popular green fluorescent reagents used for in situ labeling peptides, proteins and nucleotides. It has also been used to prepare various small fluorescent molecules.
5-FAM-X, SE contains a seven-atom aminohexanoyl spacer (known as ‘X’) between the FAM fluorophore and the succinimidyl ester. The ‘X’ spacer separates the fluorophore from the biomolecule to which it is conjugated. The ‘X’ spacer may potentially reduce the quenching that typically occurs upon conjugation. We recommend this fluorescein derivative as the preferred dye for preparing fluoresceinated proteins when the fluorescence quenching of the labeling dyes by proteins is a serious problem.

Specifications

Chemistry
CAS registry number
  • 148356-00-7
Molecular Formula
  • C31H26N2O10
Molecular Mass/ Weight
  • 586,55
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 521
Quantity & Purity
Purity
  • >98% by TLC, >97% by HPLC
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO
Storage Conditions
  • -20°C desiccated and protected from light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

The p75 neurotrophin receptor promotes amyloid-β(1-42)-induced neuritic dystrophy in vitro and in vivo.

J Neurosci . 2009 Aug 26 ; 29(34) 10627 | DOI : 10.1523/JNEUROSCI.0620-09.2009

  • J. Knowles
  • et al