Genes-analysis

BioTracker® 6X Bromophenol Blue loading dye for agarose and non-denaturing PAGE 10,000 Assays

69,00
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  • Cat.Number : EP-5010-10
  • Availability :
    In production
  • Shipping conditions : Ice delivery fees must be applied

Quantity

BioTracker® 6X is a Bromophenol Blue loading dye for agarose and non-denaturing poylacrylamide gel electrophoresis.
BioTracker® is composed of 60 % sterile DI H2O, 30 % glycerol, 10 % 10X TE, 0.25% BPB dye, and 0.75% SDS.
Tracking dye contains 25 mg/mL blue dextran of an average molecular weight of 2,000,000 dissolved in 25mM EDTA.

Sample preparation
For each sample combine 0.5µL MapMarker®, 0.5 µL of Tracking Dye, 2.5µL deionized formamide and 1-2µL of Sample. A marker-dye master mix can be prepared by combining the above components in suitable quantities for the number of samples being analyzed.

After combining samples with marker/dye/formamide mix, the mixture should be briefly vortexed followed by brief centrifugation and denatured at 95 °C for 5 min. followed by cooling to 4 °C. Load from 1-3µL of this material per lane depending on well capacity.

Specifications

Properties
Color
Quantity & Purity
Concentration
  • 10 x
Storage & stability
Form
  • In solution
Storage Conditions
  • Storage at 4°C is recommended
Activity
Usage
  • Research use

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