BioTracker® 6X is a Bromophenol Blue loading dye for agarose and non-denaturing poylacrylamide gel electrophoresis.
BioTracker® is composed of 60 % sterile DI H2O, 30 % glycerol, 10 % 10X TE, 0.25% BPB dye, and 0.75% SDS.
Tracking dye contains 25 mg/mL blue dextran of an average molecular weight of 2,000,000 dissolved in 25mM EDTA.
For each sample combine 0.5µL MapMarker®, 0.5 µL of Tracking Dye, 2.5µL deionized formamide and 1-2µL of Sample. A marker-dye master mix can be prepared by combining the above components in suitable quantities for the number of samples being analyzed.
After combining samples with marker/dye/formamide mix, the mixture should be briefly vortexed followed by brief centrifugation and denatured at 95 °C for 5 min. followed by cooling to 4 °C. Load from 1-3µL of this material per lane depending on well capacity.