PUREfrex™ is the purest cell-free protein synthesis kit based on the PURE (Protein synthesis Using Recombinant Elements) system developed by Professor Takuya Ueda (University of Tokyo). The PURE system successfully reconstituted the E.coli transcription/translation process in the test tube.
*Please mention this Special Action code: EGT-9552
*Valid until June 30st, 2012 in Austria, Benelux, Czech Republic, France, Germany, Ireland, Hungary, Poland, Slovakia, Slovenia, Switzerland and United Kingdom.
No cumulative discount can be applied.
- PURE: Reducted level of contamination
PUREfrex™ is advanced in the purity by improving the preparation method of ribosomes, tRNAs and all protein constituents compared to the original PURE system. As a result, the contaminating lipopolysaccharide [LPS] from E.coli is reduced below 0.1EU per µL of reaction [Fig.1], and other contaminants like RNase and ß-galactosidase are also reduced. Therefore, PUREfrex™ is perfectly adapted to any downstream application.
Fig.1 Lipopolysaccharides [LPS] activity detected in reaction samples using the PUREfrex™ kit vs competitor kits. The contaminating LPS activity detected in the PUREfrex™ sample is below 0.1 EU/µL.
- EASY: Any tag for your protein purification
Since PUREfrex™ protein components are free of tag, you can fuse your protein of interest to any tag to ease purification step [Fig.2].
Fig.2: His-tagged GFP production using PUREfrex™.
1: Synthesis reaction sample
2: Flowthrough sample
3: Washing step sample
4: Purified His-tagged GFP sample
- FAST: Quick protein production and purification
With PUREfrex™, obtaining the expression product of a genetic sequence only takes 2-4 hours.
FLEXIBLE: Synthesis of various proteins
Because PUREfrex™ only contains fully characterized components, it allows an easy adjustment of the assay conditions. With PUREfrex™ you can produce as easily Eukaryotic as Prokaryotic proteins [Fig.3]. With PUREfrex™, proteins can be synthesized from plasmid and PCR templates [Fig.4]
Fig.3 SDS-PAGE of Eukaryotic* and Prokaryotic** protein samples produced using PUREfrex™ : -DNA= negative control, GFP*= Green fluorescent protein, DHFR** = Dihydrofolate reductase, GST* = Glutathione S-transferase, ß-Gal** = ß-Galactosidase, bR** = Bacteriorhodopsin, Luc* = Luciferase, CS* = Citrate synthase, MDH* = Malate dehydrogenase, Her2d1* = Domain1 of Her2
Fig.4 Protein can be synthesized with PUREfrex™ from a PCR or a plasmid template.
*Please mention this Special Action code: EGT-9552
*Valid until June 30st, 2012 in Austria, Benelux, Czech Republic, France, Germany, Ireland, Hungary, Poland, Slovakia, Slovenia, Switzerland and United Kingdom.
No cumulative discount can be applied.
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