We offer RNA labelling, hybridization and data analysis services on custom made and our catologue DNA MicroArray. We provide fully analyzed data including, expression deferential, original images, and raw data tables.

 
Service procedure
 
1-Service package

Service package includes, catalogue DNA MicroArray, RNA labelling, hybridization, slide scanning, image extraction and data analysis

2- Submitting your Samples

10 micrograms of RNA samples is required and should be sent in dry ice at the minimal concentration of 1 micrograms/microliter in DEPC treated water, to Eurogentec array Department, during regular business hours, Monday to Friday, 9:00 am - 5:00 pm. The RNA samples can be either sent by express mail service or dropped by users. For expression analysis on Human Whole Genome DNA MicroArray , we require 10-20 micrograms of total RNA

3- RNA quality control

The customer will need to extract total RNA, provide a photo of a gel and the 260/280 optical density ratio to assure minimum RNA quality. Eurogentec's array Department will run RNA quality control procedure using Bioanalyzer 2100 instrument to ensure RNA quality before array experiment. If the quality is not meeting the requirement, the customers will be informed and asked to ship new RNA preparation.

 4-RNA labelling

The total RNA is retro-transcribed into cDNA; Cy3-dCTP and Cy5-dCTP are incorporated during the first strand cDNA synthesis. The labeled cDNA samples are purified and concentrated using Microcon columns (Millipore).

5-Hybridization:

3-5 micrograms per labeled total RNA sample is applied on the Human Whole Genome DNA MicroArrayr for hybridization on Array Booster(tm) station, which guarantee fast and reproducible hybridization results. The slides are washed at stringent conditions and scanned immediately on GenePix 4000B scanner, which is able to scan two wavelength excitations at 532 and 635 nm in a single phase.

6- The array results including TIFF and JPEG images of hybridization and data analysis Excel(tm) file will be recorded onto CD and sent to the customer by express mail. 

 7- Service turn around time

The array hybridization results will be returned to user within 10 working days