Hot Diamond Taq®

Hot Diamond Taq®, is a highly thermostable enzyme produced and purified from recombinant Escherichia coli bacterium containing the Thermus aquaticus DNA Polymerase gene. This thermophilic eubacterium strain lacks Taq I restriction endonuclease.

The enzyme exhibits unique Hot Start characteristics and represents a completely new “Hot Start concept”. Hot start characteristics are not accomplished through chemically modification nor a blocking antibody but a proprietary agent prevents non-specific polymerization; thereby preventing primer-dimer formation and increasing the PCR yield of specific products. 

Hot Diamond Taq® shows no amplification at room temperature and gives very high yield of specific product. The enzyme needs very short activation time (100% activated during the first PCR cycle) but is compatible with all existing protocols (from 20 sec. to 15 minutes at 95°C). This enzyme shows very good fidelity and catalyzes 5’→3’ synthesis of DNA with no detectable 3’→5’ proof reading exonuclease activity. The enzyme has the “extendase” activity allowing TA cloning.

Hot Diamond Taq® is particularly recommended for diagnostic PCR applications that require ultra low levels of bacterial & fungal DNA but also for amplification of long and difficult templates. The GMP manufacturing & purification processes minimize the risk of false positive results due to residual DNA contamination (bacterial or fungal). The enzyme is QC-tested to verify that < 1fg of genomic E.coli DNA (or 0.2 copy) is present in a standard aliquot containing 1 unit of Taq. Bioburden is guaranteed < 10 CFU/ml, but is typically = 0 CFU/ml.

Key Benefits

	Ultra-low residual DNA content (0.002 E.coli genome copies/Taq Unit).
	Pure, purity higher than 98 % and ultra-low bioburden (< 10 CFU/ml) due to production in a GMP-Pharma facility.
	Exceptional reproducibility, GMP process and stringent QC combined with rigorous analytical method ensure exceptional lot-to-lot & results reproducibility.
	Sensitive, efficiently amplifies DNA templates even at very low concentrations.
	Specific, prevents non-specific polymerization as primer-dimer formation and increasing the PCR yield of specific products
	cGMP compliant, the Quality Management System is fully compliant to the ISO13485:2003 Medical Device standard and FDA’s Quality System Regulations (21 CFR 820).
	Complete, PCR reaction buffer & MgCl2 are included.
	Customized Fill & Finish on request
	Batch reservation on request
	For Diagnostic and demanding Research PCR & qPCR
	Attractive princing

Package Content

- Hot Diamond Taq® DNA Polymerase is provided at a concentration > 5 U/µl with Hot PCR Reaction buffer 10x (750 mM Tris-HCl pH 8.8 (at 25°C), 200 mM (NH₄)₂SO₄, 0.1 % (v/v) Tween 20 and stabilizer) and MgCl₂ solution (25 mM MgCl₂).

- Hot Diamond Taq® DNA Polymerase is stored in 20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol (v/v), pH 8.0 (4°C) and stabilizer.

Shipping & Storage Conditions

- Ships on dry ice. 
- Storage at -20°C is recommended.

Documentation

For more information please dowload the Hot Diamond Taq® brochure

Literature & Resources 

	cGMP Oligo for Diagnostic kits
	cGMP Clinical Oligo for Lab Services
	Diagnostic Taq  Brochure

Regulatory 

	IVDT Article on GMP Compliance Oct_Nov 2008
	Certification ISO 13485:2003
	Certification ISO 9001:2008

Presentations 

	Presentation at AsiaTides 2009 Peter Haima, Director - Diagnostic Services
	Presentation at the EUROTIDES 2007 Conference  Quality Management System “ Interpretation and Application for GMP Manufacturing of Diagnostic Oligos” Peter Haima, Ph.D., Project Manager Diagnostics & Therapeutics
	Presentation at European Meeting on Molecular Diagnostics 2007 Quality Management in the manufacturing of primers/probes for molecular diagnostic assays Peter Haima, Ph.D., Project Manager Diagnostics & Therapeutics
	Presentation at the TIDES 2007 Conference "Interpretation and Application for Customer Manufacturing of Diagnostic Oligos"  Peter Haima, Ph.D., Project Manager Diagnostics & Therapeutics

The most convenient way to order this product is tocommunicate the following information:

	PO
	Reference
	Quantity
	Shipping address

	By email: diagnostic.taq@eurogentec.com
	By fax : +32 4 372 75 00
	By mail
	Eurogentec S.A. IVD Team LIEGE Science Park Rue du Bois Saint Jean 5 4102 Seraing BELGIUM

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