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Research Diagnostic Therapeutic

2’-Deoxy-2’-fluoro-nucleosides adopt an RNA-type sugar conformation, presumably due to the high electronegativity of fluorine.

Name	Synthesis scale	Quantity	Reference	EUR
RNA 2' Fluoro U, 1 - 10 incorporations	0.2 µmol	4 OD	OR-0071	65.00
RNA 2' Fluoro U, 1 - 10 incorporations	1.0 µmol	12 OD	OR-0072	92.50
RNA 2' Fluoro C, 1 - 10 incorporations	0.2 µmol	4 OD	OR-0075	65.00
RNA 2' Fluoro C, 1 - 10 incorporations	1.0 µmol	12 OD	OR-0076	92.50

Price per incorporation

Quality Control

MALDI-TOF Mass Spectrometry

Delivery times

10 Working days

Packaging

Lyophilized

Shipping conditions

Room temperature

Storage conditions

-20 °C to -70 °C
Oligonucleotides are stable in solution at 4 °C for up to 2 weeks. Properly reconstituted material stored at
-20 °C should be stable for at least 6 months. Lyophilized DNA (when kept at -20 °C) in a nuclease-free environment should be stable for years.

2’-Deoxy-2’-fluoro-nucleosides adopt an RNA-type sugar conformation, presumably due to the high electronegativity of fluorine. Because of this sugar conformation, RNA duplexes (A-form) are generally more thermodynamically stable than DNA duplexes (B-form). As expected, the addition of 2’-F-RNA residues to oligodeoxynucleotides progressively increases the thermal stability of their duplexes with RNA. The stabilization is additive at approximately 2° per residue. This compares favorably with 2’-OMe-RNA at around 1.5° and RNA at 1.1° per residue. In the meantime, base pair specificity remains intact.

2’-F-RNA phosphodiester linkages are not nuclease resistant, although the corresponding phosphorothioate linkages are highly resistant. Researchers usually design antisense oligonucleotides to form duplexes with RNA, which are then substrates for RNase H. Uniformly modified 2’-F-RNA/RNA duplexes are not substrates for RNase H. However, it is straightforward to prepare chimeric 2’-F-RNA/DNA phosphorothioate oligonucleotides which exhibit enhanced binding to the RNA target, are substrates for RNase H, and are highly nuclease resistant.