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2' O-Methyl modified oligonucleotides

Research Diagnostic Therapeutic
Oligo-2’ O-Methyl (O-Me) nucleotides are resistant (but not totally resistant) to a variety of ribo- and deoxyribonucleases. As well as being stable to normal handling and nuclease resistant, oligo-2’ O-Me-nucleotides form more stable hybrids with complementary RNA strands than equivalent DNA and RNA sequences.


Name Synthesis scale Quantity Purification Reference EUR
RNA 2' O-Me, 1 - 10 incorporations 0.2 µmol 4 OD PAGE OR-0031 21.90
RNA 2' O-Me, 1 - 10 incorporations 1.0 µmol 12 OD PAGE OR-0041 25.70
RNA 2' O-Me, > 10 incorporations 0.2 µmol 4 OD PAGE OR-0030 16.50
RNA 2' O-Me, > 10 incorporations 1.0 µmol 12 OD PAGE OR-0040 19.50
RNA 2' O-Me Inosine, 1 - 10 incorporations 0.2 µmol 4 OD PAGE OR-01030-02 237.00
RNA 2' O-Me Inosine, 1 - 6 incorporations 1.0 µmol 12 OD PAGE OR-01030-03 237.00
RNA 2' O-Me-5-Me-C, 1 - 10 incorporations 0.2 µmol 4 OD PAGE OR-02030-02 237.00
RNA 2' O-Me-5-Me-C, 1 - 6 incorporations 1.0 µmol 12 OD PAGE OR-02030-03 237.00
RNA 2' O-Me propylnyl-C, 1 - 10 incorporations 0.2 µmol 4 OD PAGE OR-03030-02 387.50
RNA 2' O-Me propylnyl-C, 1 - 6 incorporations 1.0 µmol 12 OD PAGE OR-03030-03 387.50
RNA 2' O-Me propylnyl-U, 1 - 10 incorporations 0.2 µmol 4 OD PAGE OR-04030-02 387.50
RNA 2' O-Me propylnyl-U, 1 - 6 incorporations 1.0 µmol 12 OD PAGE OR-04030-03 387.50
Price per incorporation

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   10 Working days

Packaging

   Lyophilised

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4 °C for up to 2 weeks. Properly reconstituted material stored at 
-20 °C should be stable for at least 6 months. Lyophilized DNA (when kept at -20 °C) in a nuclease-free environment should be stable for years.

Oligo-2’ O-Methyl (O-Me) nucleotides are resistant (but not totally resistant) to a variety of ribo- and deoxyribonucleases. As well as being stable to normal handling and nuclease resistant, oligo-2’ O-Me-nucleotides form more stable hybrids with complementary RNA strands than equivalent DNA and RNA sequences. This combination of useful properties promises to make 2’ O-Me-RNA sequences powerful research tools. Conveniently, the synthesis and handling of 2’ O-Me RNA is as simple to perform as DNA synthesis, and avoids most of the problems associated with RNA synthesis.

The combination of properties offered by oligo-2’ O-Me-nucleotides (nuclease resistance, stable hybrid formation, ease of synthesis) make them especially suitable for use as antisense probes. In this mode, RNase H resistant oligo-2’ O-Me-nucleotides, labelled or unlabelled, can be used to block specific RNA functions and, when attached to a suitable tag, usually biotin, can be used in affinity purification of RNA complexes.


Legal notices

   For Research Use only

Leaflets

Product citations


FARIA M. et al., "Phosphoramidate oligonucleotides as potent antisense molecules in cells and in vivo", Nature Biotechnology, vol. 19, n° 1, p. 40-44, January 2001


DE BACKER M. et al., "An antisense-based functional genomics approach for identification of genes critical for growth of Candida albicans", Nature Biotechnology, vol. 19, n° 3, p. 235-241, March 2001


ANGO F. et al., "Agonist-independent activation of metabotropic glutamate receptors by the intracellular protein Homer", Nature, n° 411, p. 962-965, 21 June 2001


VAN HEUSDEN J. et al., "Inhibition of all-TRANS-retinoic acid metabolism by R116010 induces antitumour activity", British Journal of Cancer, vol. 86, n° 4, p. 605-611, 12 February 2002


SKORDIS L. et al., "Bifunctional antisense oligonucleotides provide a trans-acting plicing enhancer that stimulates SMN2 gene expression in paient fibroblasts", PNAS, vol. 100, n° 7, p. 4114-4119, April 2003


GROTE K. et al., "Stretch-inducible Expression of the Angiogenic Factor CCN1 in Vascular Smooth Muscle Cells Is Mediated by Egr-1", Journal of Biological Chemistry, vol. 279, n° 53, p. 55675-55681, December 2004





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