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2' O-Methyl RNA Mole... Build & Order

It appears that linear 2' O-Methyl RNA Molecular Beacons are usually more suitable for specific detection of these RNAs, representing different classes of RNA, in the nuclei of living cells.

Name	Synthesis scale	Recommended Purification*	Included Purification	Reference	EUR
2’O-Me Molecular Beacon 5’ FAM + 3’ DABCYL	200 nmol	N/A	Dual HPLC (RP+RP)	PB-OB201-020	744.60
2’O-Me Molecular Beacon 5’ HEX + 3’ DABCYL	200 nmol	N/A	Dual HPLC (RP+RP)	PB-OB210-020	744.60
2’O-Me Molecular Beacon 5’ TET + 3’ DABCYL	200 nmol	N/A	Dual HPLC (RP+RP)	PB-OB212-020	744.60

* More info on the Purifications page

Quality Control

MALDI-TOF Mass Spectrometry and Analytical HPLC

Delivery times

15-39 bases: 12 Working days
40-80 bases: 15 Working days

Packaging

Lyophilised

Shipping conditions

Room temperature

Storage conditions

-20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Lyophilized DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

To detect the various RNA classes in living cells, several approaches have been developed. One of these is based on the use of 2' O-Methyl RNA probes for the detection of small nuclear RNAs. 2' O-Methyl RNA probes are considered to perform better then DNA oligonucleotides because they are not only nuclease resistant, but also possess a higher affinity, increased specificity, faster hybridization kinetics, and a superior ability to bind to structured targets compared to DNA oligonucleotides. Recently, Molecular Beacons were introduced for RNA detection in living cells. The rationale for using Molecular Beacons to detect RNAs in living cells was to improve signal-to-noise ratios by eliminating fluorescence signals derived from non-hybridized probe sequences. It appears that linear 2' O-Methyl RNA Molecular Beacons are usually more suitable for specific detection of these RNAs, representing different classes of RNA, in the nuclei of living cells. Molecular Beacons result in images with improved signal-to-noise ratios, thereby leading to better detection sensitivity.

Legal notices

For Research Use only

Leaflets

Product citations

GIESENDORF B. et al., "Molecular beacons: a new approach for semiautomated mutation analysis", Clinical Chemistry, vol. 44, n° 3, p. 482-486, 1998

NILSSON M. et al., "Real-Time monitoring of rolling-circle amplification using a modified molecular beacon design", Nucleic Acids Research, vol. 30, n° 14, May 2002

KEHLENBACH R. et al., "In vitro analysis of nuclear mRNA export using molecular beacons for target detection", Nucleic Acids Research, vol. 31, n° 11, April 2003

TIMM J. et al., "Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients", PNAS, vol. 100, n°24, p. 14321-14326, November 2003

DAWES S. et al., "Ribonucleotide Reduction in Mycobacterium tuberculosis: Function and Expression of Genes Encoding Class Ib and Class II Ribonucleotide Reductases", Infection and Immunity, vol. 71, n° 11, p. 6124-6131, November 2003

DAHL F. et al., "Circle-to-circle amplification for precise and sensitive DNA analysis", PNAS, vol. 101, n° 13, p. 4548-4553, March 2004

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