Home > Products > N > 3' DABCYL Molecular Beacons
Back to previous page

3' DABCYL Molecular Beacons

Life Science Molecular Diagnostics GMP BioManufacturing
3' DABCYL Molecular ... Build & Order
Eurogentec is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and quenchers. All Molecular Beacons are provided double-HPLC purified. The length of Molecular Beacons with a 3’ DABCYL should be comprised between 25 to 45 bases.


Name Synthesis scale Recommended Purification* Included Purification Reference EUR
Molecular Beacon 5’ FAM + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB201-004 343.00
Molecular Beacon 5’ FAM + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB201-020 464.10
Molecular Beacon 5’ FAM + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB201-100 550.80
Molecular Beacon 5’ HEX + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB210-004 343.00
Molecular Beacon 5’ HEX + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB210-020 464.10
Molecular Beacon 5’ HEX + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB210-100 550.80
Molecular Beacon 5’ TET + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB212-004 343.00
Molecular Beacon 5’ TET + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB212-020 464.10
Molecular Beacon 5’ TET + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB212-100 550.80
Molecular Beacon 5’ JOE + 3’ DABCYL 40 nmol N/A IEX-HPLC PB-MB214-004 433.00
Molecular Beacon 5’ JOE + 3’ DABCYL 200 nmol N/A IEX-HPLC PB-MB214-020 535.50
Molecular Beacon 5’ JOE + 3’ DABCYL 1000 nmol N/A IEX-HPLC PB-MB214-100 632.40
Molecular Beacon 5’ ROX + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB216-004 480.00
Molecular Beacon 5’ ROX + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB216-020 663.00
Molecular Beacon 5’ ROX + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB216-100 877.20
Molecular Beacon 5’ Rhodamine-6G + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB220-004 480.00
Molecular Beacon 5’ Rhodamine-6G + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB220-020 663.00
Molecular Beacon 5’ Rhodamine-6G + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB220-100 877.20
Molecular Beacon 5’ TAMRA + 3 ‘DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB200-004 343.00
Molecular Beacon 5’ TAMRA + 3 ‘DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB200-020 464.10
Molecular Beacon 5’ TAMRA + 3 ‘DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB200-100 550.80
Molecular Beacon 5’ Texas Red® + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB230-004 343.00
Molecular Beacon 5’ Texas Red® + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB230-020 464.10
Molecular Beacon 5’ Texas Red® + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB230-100 550.80
Molecular Beacon 5’ Yakima Yellow®  + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB240-004 370.00
Molecular Beacon 5’ Yakima Yellow®  + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB240-020 530.00
Molecular Beacon 5’ Yakima Yellow®  + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB240-100 742.10
Molecular Beacon 5’ Dragonfly Orange™ + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB245-004 480.00
Molecular Beacon 5’ Dragonfly Orange™ + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB245-020 663.00
Molecular Beacon 5’ Dragonfly Orange™ + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB245-100 877.20
Molecular Beacon 5’ Cy®  dyes + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB250-004 350.00
Molecular Beacon 5’ Cy®  dyes + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB250-020 464.00
Molecular Beacon 5’ Cy®  dyes + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB250-100 550.00
Molecular Beacon 5’ Alexa® + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB260-004 550.00
Molecular Beacon 5’ Alexa® + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB260-020 663.00
Molecular Beacon 5’ Alexa® + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB260-100 877.00
Molecular Beacon 5’ Cascade Blue® + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB270-004 480.00
Molecular Beacon 5’ Cascade Blue® + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB270-020 663.00
Molecular Beacon 5’ Cascade Blue® + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB270-100 877.20
Molecular Beacon 5’ DYOMICS + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB280-004 On Request
Molecular Beacon 5’ DYOMICS + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB280-020 On Request
Molecular Beacon 5’ DYOMICS + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB280-100 On Request
Molecular Beacon 5’ BODIPY® + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB285-004 On Request
Molecular Beacon 5’ BODIPY® + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB285-020 On Request
Molecular Beacon 5’ BODIPY® + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB285-100 On Request
Molecular Beacon 5’ ATTO + 3’ DABCYL 40 nmol N/A Dual HPLC (RP+RP) PB-MB290-004 On Request
Molecular Beacon 5’ ATTO + 3’ DABCYL 200 nmol N/A Dual HPLC (RP+RP) PB-MB290-020 On Request
Molecular Beacon 5’ ATTO + 3’ DABCYL 1000 nmol N/A Dual HPLC (RP+RP) PB-MB290-100 On Request
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry and Analytical HPLC

Delivery times

   15-39 bases: 12 Working days
                             40-80 bases: 15 Working days

Packaging

   Lyophilised

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Lyophilized DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

Molecular Beacons are probes which contain a stem-loop structure, a fluorophore and a quencher at their 5’ and 3’ ends, respectively.

The ‘stem’ sequence keeps the fluorophore and the quencher together, but only in the absence of a sequence complementary to the ‘loop’ sequence. As long as the fluorophore and the quencher are in close vicinity, any photons emitted by the fluorophore are absorbed by the quencher. This phenomenon is called collisional (or proximal) quenching.

In the presence of a complementary sequence, the probe unfolds and hybridizes to the target, the fluorophore is displaced from the quencher, which can no longer absorb the photons emitted by the fluorophore, and the probe starts to fluoresce. The amount of signal is proportional to the amount of target sequence, and is measured in Real-Time to allow quantification of the amount of target sequence.

Eurogentec is a licensed supplier of Molecular Beacons, and offers a large number of fluorescent reporters and quenchers. All Molecular Beacons are provided double-HPLC purified and controlled by analytical HPLC and MALDI-TOF MS. The maximum length of standard Molecular Beacons with a 3’ DABCYL is 45 bases. Molecular Beacons are provided lyophilized in individual tubes.

Eurogentec determines upon request the signal-to-noise ratio (STNR) for each Molecular Beacons by hybridizing it to its complementary sequence and comparing fluorescence to background. For FAM labelled Molecular Beacons, the STNR should be > 15. All other dyes require a signal-to-noise ratio > 10. Eurogentec proposes two specialty products: Wavelength-Shifting Molecular Beacons and 2’ O-Methyl RNA Molecular Beacons.

Legal notices

   For Research Use only

Order Forms

Leaflets

Product citations


GIESENDORF B. et al., "Molecular beacons: a new approach for semiautomated mutation analysis", Clinical Chemistry, vol. 44, n° 3, p. 482-486, 1998


NILSSON M. et al., "Real-Time monitoring of rolling-circle amplification using a modified molecular beacon design", Nucleic Acids Research, vol. 30, n° 14, May 2002


KEHLENBACH R. et al., "In vitro analysis of nuclear mRNA export using molecular beacons for target detection", Nucleic Acids Research, vol. 31, n° 11, April 2003


TIMM J. et al., "Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients", PNAS, vol. 100, n°24, p. 14321-14326, November 2003


DAWES S. et al., "Ribonucleotide Reduction in Mycobacterium tuberculosis: Function and Expression of Genes Encoding Class Ib and Class II Ribonucleotide Reductases", Infection and Immunity, vol. 71, n° 11, p. 6124-6131, November 2003


DAHL F. et al., "Circle-to-circle amplification for precise and sensitive DNA analysis", PNAS, vol. 101, n° 13, p. 4548-4553, March 2004


  • Online ordering: The most convenient way to order your oligonucleotides, peptides and Real-Time qPCR kits is to use our web ordering system. To place orders online, simply login if you have already an online account.

    New online customers ? Not registered yet ? Request your free login and password.

  • By e-mail: order@eurogentec.com
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

To receive relevant information on our products, you should first select your country :
Country selection page