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Amino modifiers

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Amino modifiers Build & Order
The introduction of a primary Amine (NH2) at the 5’-position is used to functionalize the corresponding terminus of the nucleotide for conjugation with e.g. an activated NHS ester or isothiocyanate fluorescent label. Several spacers are available, all of them are hydrophobic. The available lengths are 3, 6, or 12 methylene (CH2) groups between the terminal phosphate and the amino “moiety”.

Name Synthesis scale Recommended Purification* Required Purification* Reference EUR
5' Amino Modifier C3 40 nmol RP-HPLC - MD-MF003-05004 18.00
5' Amino Modifier C3 200 nmol RP-HPLC - MD-MF003-05020 29.00
5' Amino Modifier C3 1000 nmol RP-HPLC - MD-MF003-05100 47.70
5' Amino Modifier C6 40 nmol RP-HPLC - MD-MF006-05004 20.10
5' Amino Modifier C6 200 nmol RP-HPLC - MD-MF006-05020 31.20
5' Amino Modifier C6 1000 nmol RP-HPLC - MD-MF006-05100 49.80
5' Amino Modifier C6 2.5 µmol RP-HPLC - MD-MF006-05M02 121.70
5' Amino Modifier C6 5 µmol RP-HPLC - MD-MF006-05M05 189.90
5' Amino Modifier C6 10 µmol RP-HPLC - MD-MF006-05M10 343.40
5' Amino Modifier C12 40 nmol RP-HPLC - MD-MF012-05004 38.00
5' Amino Modifier C12 200 nmol RP-HPLC - MD-MF012-05020 47.70
5' Amino Modifier C12 1000 nmol RP-HPLC - MD-MF012-05100 78.00
3' Amino Modifier C6 40 nmol RP-HPLC - MD-MF006-03004 24.90
3' Amino Modifier C6 200 nmol RP-HPLC - MD-MF006-03020 41.00
3' Amino Modifier C6 1000 nmol RP-HPLC - MD-MF006-03100 53.00
Internal Amino Modifier C6 (dT) 40 nmol RP-HPLC - MD-MF006-DT004 94.00
Internal Amino Modifier C6 (dT) 200 nmol RP-HPLC - MD-MF006-DT020 123.00
Internal Amino Modifier C6 (dT) 1000 nmol RP-HPLC - MD-MF006-DT100 157.00
Internal Amino Modifier Propargyl-dU 40 nmol RP-HPLC - MD-MF030-IN004 257.00
Internal Amino Modifier Propargyl-dU 200 nmol RP-HPLC - MD-MF030-IN020 324.50
Internal Amino Modifier Propargyl-dU 1000 nmol RP-HPLC - MD-MF030-IN100 417.00
Internal Amine (dR) 40 nmol RP-HPLC - MD-MF040-DR004 51.00
Internal Amine (dR) 200 nmol RP-HPLC - MD-MF040-DR020 62.60
Internal Amine (dR) 1000 nmol RP-HPLC - MD-MF040-DR100 95.10
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                             15-39 bases: 5 Working days
                             40-80 bases: 7-8 Working days
                             > 80 bases: 10 Working days

Packaging

   Dried

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
The introduction of a primary Amine (NH2) at the 5’-position is used to functionalize the corresponding terminus of the nucleotide for conjugation with e.g. an activated NHS ester or isothiocyanate fluorescent label. Several spacers are available, all of them are hydrophobic. The available lengths are 3, 6, or 12 methylene (CH2) groups between the terminal phosphate and the amino “moiety”. 



The 3’-NH2 modification allows the prevention of the 3’>5’ exonuclease degradation of oligonucleotides in biological fluids such as cell culture medium.


The following amino modifiers C6-dT, dU, Propargyl-dU and Propargylcaproyl-dU are used in post-coupling reactions, for instance, for modifying oligonucleotides that will be printed onto microarrays.




Finally, the dR-NH2 modification (deoxyribose-NH2) which forms abasic sites in the oligonucleotide, provides the possibility to add more than one label anywhere in the sequence or on either terminus after post-coupling reactions between the Amine group and an activated label. The label is linked to the deoxyribose via a 6-carbon atom spacer which reduces steric hindrance.


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Product citations


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PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002


OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002


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KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002


VANDEN ABEELE F. et al., "Store-operated Ca2+ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003


GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N4-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004


MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004


DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004



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