ATTO-TEC dyes can be used as fluorescent labels for a large variety of biomolecules including oligonucleotides. ATTO dyes cover the spectral region from 350 nm in the UV to 750 nm in the near infrared.
| Name |
Synthesis scale |
Recommended Purification* |
Required Purification* |
Reference |
EUR |
|
| 5’ ATTO 390 |
40 nmol
|
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-39005004 |
175.00 |
|
| 5’ ATTO 390 |
200 nmol
|
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-39005020 |
221.00 |
|
| 5’ ATTO 390 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-39005100 |
290.00 |
|
| 5’ ATTO 425 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-42505004 |
175.00 |
|
| 5’ ATTO 425 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-42505020 |
221.00 |
|
| 5’ ATTO 425 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-42505100 |
290.00 |
|
| 5’ ATTO 465 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-46505004 |
175.00 |
|
| 5’ ATTO 465 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-46505020 |
221.00 |
|
| 5’ ATTO 465 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-46505100 |
290.00 |
|
| 5’ ATTO 488 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-48805004 |
175.00 |
|
| 5’ ATTO 488 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-48805020 |
221.00 |
|
| 5’ ATTO 488 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-48805100 |
290.00 |
|
| 5’ ATTO 495 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-49505004 |
175.00 |
|
| 5’ ATTO 495 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-49505020 |
221.00 |
|
| 5’ ATTO 495 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-49505100 |
290.00 |
|
| 5’ ATTO 520 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-52005004 |
175.00 |
|
| 5’ ATTO 520 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-52005020 |
221.00 |
|
| 5’ ATTO 520 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-52005100 |
290.00 |
|
| 5’ ATTO 532 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-53205004 |
175.00 |
|
| 5’ ATTO 532 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-53205020 |
221.00 |
|
| 5’ ATTO 532 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-53205100 |
290.00 |
|
| 5’ ATTO 550 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-55005004 |
175.00 |
|
| 5’ ATTO 550 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-55005020 |
221.00 |
|
| 5’ ATTO 550 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-55005100 |
290.00 |
|
| 5’ ATTO 565 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-56505004 |
175.00 |
|
| 5’ ATTO 565 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-56505020 |
221.00 |
|
| 5’ ATTO 565 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-56505100 |
290.00 |
|
| 5’ ATTO 590 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-59005004 |
175.00 |
|
| 5’ ATTO 590 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-59005020 |
221.00 |
|
| 5’ ATTO 590 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-59005100 |
290.00 |
|
| 5’ ATTO 594 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-59405004 |
175.00 |
|
| 5’ ATTO 594 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-59405020 |
221.00 |
|
| 5’ ATTO 594 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-59405100 |
290.00 |
|
| 5’ ATTO 610 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-61005004 |
On Request |
|
| 5’ ATTO 610 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-61005020 |
On Request |
|
| 5’ ATTO 610 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-61005100 |
On Request |
|
| 5’ ATTO 620 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-62005004 |
175.00 |
|
| 5’ ATTO 620 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-62005020 |
221.00 |
|
| 5’ ATTO 620 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-62005100 |
290.00 |
|
| 5’ ATTO 633 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63305004 |
On Request |
|
| 5’ ATTO 633 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63305020 |
On Request |
|
| 5’ ATTO 633 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63305100 |
On Request |
|
| 5’ ATTO 635 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63505004 |
On Request |
|
| 5’ ATTO 635 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63505020 |
On Request |
|
| 5’ ATTO 635 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63505100 |
On Request |
|
| 5’ ATTO 637 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63705004 |
On Request |
|
| 5’ ATTO 637 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63705020 |
On Request |
|
| 5’ ATTO 637 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-63705100 |
On Request |
|
| 5’ ATTO 647N |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-64705004 |
175.00 |
|
| 5’ ATTO 647N |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-64705020 |
221.00 |
|
| 5’ ATTO 647N |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-64705100 |
290.00 |
|
| 5’ ATTO 655 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-65505004 |
175.00 |
|
| 5’ ATTO 655 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-65505020 |
221.00 |
|
| 5’ ATTO 655 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-65505100 |
290.00 |
|
| 5’ ATTO 680 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-68005004 |
175.00 |
|
| 5’ ATTO 680 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-68005020 |
221.00 |
|
| 5’ ATTO 680 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-68005100 |
290.00 |
|
| 5’ ATTO 700 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-70005004 |
175.00 |
|
| 5’ ATTO 700 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-70005020 |
221.00 |
|
| 5’ ATTO 700 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-70005100 |
290.00 |
|
| 5’ ATTO 725 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-72505004 |
175.00 |
|
| 5’ ATTO 725 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-72505020 |
221.00 |
|
| 5’ ATTO 725 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-72505100 |
290.00 |
|
| 5’ ATTO 740 |
40 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-74005004 |
175.00 |
|
| 5’ ATTO 740 |
200 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-74005020 |
221.00 |
|
| 5’ ATTO 740 |
1000 nmol |
Dual HPLC (RP+RP) |
RP-HPLC |
MD-FL090-74005100 |
290.00 |
|
* More info on the Purifications pageQuality Control MALDI-TOF Mass Spectrometry and Analytical HPLC Delivery times 2-14 bases: 5 Working days 15-39 bases: 5 Working days 40-80 bases: 7-8 Working days > 80 bases: 10 Working days Packaging Dried Shipping conditions Room temperature Storage conditions -20 °C to -70 °C Oligonucleotides are stable in solution at 4 °C for up to 2 weeks. Properly reconstituted material stored at -20 °C should be stable for at least 6 months. Dried DNA (when kept at -20 °C) in a nuclease-free environment should be stable for years.
ATTO-TEC dyes can be used as fluorescent labels for a large variety of biomolecules including oligonucleotides. ATTO dyes cover the spectral region from 350 nm in the UV to 750 nm in the near infrared. ATTO dyes main features are : - Strong absorption
- High fluorescence quantum yield
- High photostability
- Good water solubility
- Low triplet formationIdeally suited for bioanalytical applications
ATTO-TEC fluorescent labels are ideal for many bio-analytical applications. The absorption and fluorescence of some dyes are optimized for the red region of the spectrum, with the advantage of efficiently suppressing or by-passing any auto-fluorescence of biological samples. Other dyes exhibit a large shift of fluorescence relative to the absorption (Stokes-Shift). Again others are very hydrophilic, thus avoiding non-specific adsorption at bio-molecules and surfaces.
ATTO-TEC labels also find many applications in fluorescence spectroscopy. All dyes are fully characterized and can be used in time resolved spectroscopy, FRET (Fluorescence Resonance Energy Transfer) Real-Time qPCR assays applications as well as single- molecule detection. - ATTO 520 and ATTO 532 are fluorescent labels related to the well-known dye Rhodamine-6G.
- ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B is the commercial alternative to NEDTM.
- ATTO 565, ATTO 590 and ATTO 594 are fluorescent labels belonging to the class of Rhodamine dyes. ATTO 594 is an alternative to Alexa Fluor® 594. ATTO 594 exhibits excellent water solubility, very good stability over a wide pH range, very good photo-stability, and high fluorescence quantum yield.
- ATTO 610, ATTO 611X, ATTO 620, ATTO 633, ATTO 635, ATTO 637, ATTO 647 and ATTO 647N are fluorescent labels for the red spectral region.
- ATTO 633 is an alternative to Alexa Fluor 633, ATTO 633 shows strong absorption, very high fluorescence quantum yield, and very good stability in the pH-range 2 to 10.
- ATTO 637 is another alternative to Alexa Fluor 633. Although very similar to ATTO 635, ATTO 637 is more hydrophilic and better soluble in water.
- ATTO 647N is an alternative to Cy® 5 and Alexa Fluor® 647. The characteristic features of ATTO 647N are strong absorption, extraordinary high fluorescence quantum yield and outstanding pH and photo-stability.
- Suitable excitation sources for ATTO 637 are the 633 nm line of the He:Ne Laser or a Diode-Laser emitting at 635 nm. ATTO 647 and ATTO 647N are excited most efficiently in the range 615 - 660 nm. A suitable excitation source, for instance, is the 647 nm line of the Krypton-Ion laser or a Diode-Laser emitting at 650 nm.
- ATTO 655, ATTO 680 and ATTO 700 belong to a new generation of fluorescent labels. Their fluorescence is excited most efficiently in the range 625 - 660 nm, 645 - 695 nm and 665
- 715 nm respectively. A suitable excitation source for these dyes is the Krypton-Ion laser using the 676 nm line or a Diode-Laser emitting at 670 nm.
- ATTO 725 together with ATTO 740 are fluorescent labels for the near infrared spectral region. Their fluorescence is excited most efficiently in the range 700 - 745 nm and 720 - 755 nm respectively.
- ATTO 725 shows strong absorption and fluorescence. It can be excited with long-wavelength diode lasers.
- ATTO 740 is another of our new near-infrared emitting fluorophores.Its features are strong absorption and acceptable fluorescence.
Legal notices For Research Use only | Colour | Commonly used dyes | Possible substitutes | ATTO dyes | Cy dyes | Alexa dyes | Other dyes | | Blue | | | | Alexa 405 | Pacific Blue™
Cascade Blue™ | | Green | 6-FAM, FITC | ATTO 488* | Cy® 2 | Alexa 488 | Oregon Green | | Yellow | TET | ATTO 520 | | | CAL Fluor Gold 540 | | Yellow | JOE, HEX1 | ATTO 532* | | Alexa 532 | CAL Fluor Orange 560
Bodipy 530/550 | | Yellow | VIC™ | | | | Yakima Yellow* | | Yellow | NED™ | | | | Dragonfly Orange*
Quasar 570 | | Orange | | ATTO 550 | Cy® 3 | Alexa 555
Alexa 5462 | Bodipy 558/568
DY-547
DY-555 | | Orange | TAMRA | ATTO 550 | | | Bodipy 558/568
CAL Fluor Red 590 | | Orange | ROX | ATTO 565* | Cy® 3.5 | Alexa 594 | Bodipy 558/568
LCRed 610
CAL Fluor Red 610 | | Red | Texas Red® | ATTO 590*
ATTO 5943 | | Alexa 594 | LCRed 610
CAL Fluor Red 610 | | Red | | ATTO 633
ATTO 635 | | Alexa 633 | Bodipy 630/650 | | Red | | ATTO 635 | | Alexa 635 | | | Red | | ATTO 647N
ATTO 6554 | Cy® 5 | Alexa 647 | Quasar 670
DY-647
DY-650 | | Red | | ATTO 680
ATTO 700 | Cy® 5.5 | Alexa 680
Alexa 7005 | Quasar 705
DY-680
LCRed 705 | | Far red | | | Cy® 7 | Alexa 750 | DY-732 | | Far red | | | | | IRD800
DY-780
DY-781 |
* Experimentally validated alternatives
1 Those dyes are also similar to VIC™
2 Second best Alexa alternative for Cy® 3
3 Second best ATTO alternative for Texas Red®
4 Second best ATTO alternative for Cy® 5
5 Second best Alexa alternative for Cy® 5.5
LeafletsProduct citationsLEGENDRE D. et al., "Engineering a regulatable enzyme for homogeneous immunoassays", Nature Biotechnology, vol. 17, n° 1, p.67-72, 1 January 1999 MOMENI P. et al., "Mutations in a new gene, encoding a zinc-finger protein, cause tricho-rhino-phalangeal syndrome type I", Nature Genetics, vol. 24, p. 71-74, 1 January 2000 PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002 OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002 GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004 RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004 SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005
VAN DER STEEGE G. et al., "Persistent failures in gene repair", Nature Biotechnology, n° 19, p. 305-306, April 2001 ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002 KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002 VANDEN ABEELE F. et al., "Store-operated Ca2+ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003 GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N4-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004 MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004 DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004
CHEN J. et al., "Activity-Induced Expression of Common Reference Genes in Individual CNS Neurons", Laboratory Investigation, vol. 81, n°6, p. 913-916, June 2001 VERLINDEN L. et al., "Previtamin D3 with a trans-Fused Decalin CD-ring Has Pronounced Genomic Activity", The Journal of Biological Chemistry, vol. 278, n°37, p. 35476-35482, September 2003 CHARBONNIER Y. et al., "A generic approach for the design of whole-genome oligoarrays, validated for genomotyping, deletion mapping and gene expression analysis on Staphylococcus aureus", BMC Genomics, 17;6:95, June 2005 EBNER K. et al., "Molecular Detection and Quantitative Analysis of the Entire Spectrum of Human Adenoviruses by a Two-Reaction Real-Time PCR Assay", Journal of Clinical Microbiology, vol. 43, n° 7, p. 3049-3053, July 2005 GIESENDORF B. et al., "Molecular beacons: a new approach for semiautomated mutation analysis", Clinical Chemistry, vol. 44, n° 3, p. 482-486, 1998 NILSSON M. et al., "Real-Time monitoring of rolling-circle amplification using a modified molecular beacon design", Nucleic Acids Research, vol. 30, n° 14, May 2002 KEHLENBACH R. et al., "In vitro analysis of nuclear mRNA export using molecular beacons for target detection", Nucleic Acids Research, vol. 31, n° 11, April 2003 TIMM J. et al., "Differential expression of iron-, carbon-, and oxygen-responsive mycobacterial genes in the lungs of chronically infected mice and tuberculosis patients", PNAS, vol. 100, n°24, p. 14321-14326, November 2003 DAWES S. et al., "Ribonucleotide Reduction in Mycobacterium tuberculosis: Function and Expression of Genes Encoding Class Ib and Class II Ribonucleotide Reductases", Infection and Immunity, vol. 71, n° 11, p. 6124-6131, November 2003 DAHL F. et al., "Circle-to-circle amplification for precise and sensitive DNA analysis", PNAS, vol. 101, n° 13, p. 4548-4553, March 2004 THELWELL N. et al., "Mode of action and application of Scorpion primers to mutation detection", Nucleic Acids Research, vol. 28, n° 19, p. 3752-3761, October 2000 HART K.W. et al., "Novel method for detection, typing, and quantification of human papillomaviruses in clinical samples", Journal of Clinical Microbiology, vol. 39, n° 9, p. 3204-3212, September 2001 SOLINAS A. et al., "Duplex Scorpion primers in SNP analysis and FRET applications", Nucleic Acids Research, vol. 29, n° 20, October 2001 SCHENA L. et al., "Identification and Detection of Rosellinia Necatrix by Conventional and Real-time Scorpion-PCR", European Journal of Plant Pathology, vol. 108, n° 4, p. 355-366(12), May 2002 SCHENA L. et al., "Molecular Detection of Strain L47 of Aureobasidium pullulans, a Biocontrol Agent of Postharvest Diseases", Plant Disease, vol. 86, n° 1, 54-60, 2002 SCHENA L. and IPPOLITO A., "Rapid and sensitive detection of Rosellinia necatrix in roots and soils by Real-Time Scorpions-PCR", Journal of Plant Pathology, 85 (1), 15-25, January 2003 IPPOLITO A. et al., "Real-Time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil", European Journal of Plant Pathology, vol. 110, 833-843, 2004 SCHENA L. et al., "Real-Time quantitative PCR: a new technology to detect and study phytopathogenic and antagonistic fungi", European Journal of Plant Pathology, vol. 110, 893-908, 2004
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- By e-mail: order@eurogentec.com
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