Authentik™ DNA polymerase is a heat-activated high-performance proprietary complex of enzymes specifically designed for low-copy templates or challenging PCR assays which require both high processivity and high-fidelity. Authentik™ provides improved specificity and very high PCR sensitivity, thereby eliminating the presence of non-specifics such as primer-dimers and mis-primed products. Authentik™ is inactive at room temperature and therefore requires activation by heat treatment for 10 minutes at 95 °C prior to the PCR reaction being performed.
Authentik™ DNA Polymerase is recommended for short Genomic DNA fragments of up to 3 kb, or up to 5 Kb on Lambda DNA. Authentik™ is ideal for direct cloning without the need to verify the sequence prior to expression. Authentik™ possesses 5’g3’ polymerase activity and 3’g5’ proofreading activity, which in combination with other properties, provides higher fidelity than Taq.
The specificity and performance of Authentik™ DNA Polymerase can be further improved with the use of the Booster Mix, which is designed for GC or AT rich DNA sequences.
| A 2-fold serial dilution of template lambda DNA (5 Kb) was performed from 0.25 ng (lane 1) to 31.25 pg (lane 4)
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 | A 2-fold serial dilution of template human fragment of ß actin gene (800 bp) was performed from 50 ng (lane 1) to
390 pg (lane 8)
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