Benzonase® is a genetically engineered endonuclease, which degrades both DNA and RNA strands in many forms to small oligonucleotides. It promotes quick reduction of the viscosity of cell lysates, which facilitates ultracentrifugation and increases the capacity and life of chromatography columns. It saves time, reducing proteolysis and increasing the yield in targeted protein and offers complete elimination of nucleic acids from recombinant proteins.
Quality Control
Tested for the absence of proteases.
Unit definition
The amount of enzyme that will release 1 nmol of acid-soluble total nucleotide in 30 minutes at 37 °C.
Notes
Due to the exceptionally high activity of Benzonase (400000 U/mg), the maximum concentration required is 1 µg/ml. Benzonase® is stable for seven days at 20 °C.
Concentration
25 U/µl