Booster Mix is a special 2x additive for use in reactions involving any thermostable DNA polymerase, and is designed to dramatically improve reaction specificity. Booster mix provides an optimized composition of reagents, and is ideally suited to dirty/difficult templates with GC or AT-rich DNA, repetitive sequences or difficult melting profiles.
Booster Mix acts as a melting agent by facilitating the DNA polymerase and oligonucleotides access to the template DNA. Booster Mix does not contain magnesium, dNTPs, or buffer components. In some cases it may be necessary to optimize the magnesium concentration.