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C5-methyl analogues

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C5-methyl analogues Build & Order
C-5 methylated pyrimidine deoxynucleosides are known to form more stable duplexes and triplexes than their corresponding pyrimidine derivatives.

Consequently, thymidine and 5-methyl-2’-deoxyCytidine (5-Me-dC) form more stable complexes than             2’-deoxyUridine and 2’-deoxyCytidine (dC).

Name Synthesis scale Recommended Purification* Required Purification* Reference EUR
3' (5-Me dC) 40 nmol RP-HPLC - MD-NB152-03004 55.00
3' (5-Me dC) 200 nmol RP-HPLC - MD-NB152-03020 65.00
3' (5-Me dC) 1000 nmol RP-HPLC - MD-NB152-03100 101.50
5-Me dC 40 nmol RP-HPLC - MD-NB152-IN004 38.00
5-Me dC 200 nmol RP-HPLC - MD-NB152-IN020 45.00
5-Me dC 1000 nmol RP-HPLC - MD-NB152-IN100 57.00
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days 
                             15-39 bases: 5 Working days
                             40-80 bases: 7-8 Working days 
                             > 80 bases: 10 Working days 

Packaging

   Dried

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to –70 °C
Oligonucleotides are stable in solution at 4 °C for up to 2 weeks. Properly reconstituted material stored at -20 °C should be stable for at least 6 months.Dried DNA (when kept at -20 °C) in a nuclease-free environment should be stable for years.
C-5 methylated pyrimidine deoxynucleosides are known to form more stable duplexes and triplexes than their corresponding pyrimidine derivatives.

Consequently, thymidine and 5-methyl-2’-deoxyCytidine (5-Me-dC) form more stable complexes than 2’-deoxyUridine and 2’-deoxyCytidine (dC).

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   For Research Use only

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Product citations


LEGENDRE D. et al., "Engineering a regulatable enzyme for homogeneous immunoassays", Nature Biotechnology, vol. 17, n° 1, p.67-72, 1 January 1999


MOMENI P. et al., "Mutations in a new gene, encoding a zinc-finger protein, cause tricho-rhino-phalangeal syndrome type I", Nature Genetics, vol. 24, p. 71-74, 1 January 2000


PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002


OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002


GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004


RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004


SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005


VAN DER STEEGE G. et al., "Persistent failures in gene repair", Nature Biotechnology, n° 19, p. 305-306, April 2001


ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002


KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002


VANDEN ABEELE F. et al., "Store-operated Ca2+ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003


GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N4-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004


MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004


DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004



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