Clear-MiR™ miRNA inhibitors are chemically modified antisense RNA oligonucleotides optimized to specifically target miRNA molecules in cells. 

Such antisense molecules have been shown to inhibit very efficiently the activities of specific miRNAs (Krüztfeldt et al., 2005, 2007). Such an inhibition (or knock down) is correlated to an increase of the target gene(s) activity and allows the generation of loss-of-function mutants.

Clear-MiR™ miRNA inhibitors are available in two different formats called Clear-MiR™ (for cell culture knock down experiments) and Clear-MiR™ Plus (for in vivo knock down experiments).

Name	Quantity	Purification	Reference	EUR
Clear-MiR™ miRNA inhibitors	5 nmol	PAGE	OR-0032-XXX*	240.00

* Unique identifier corresponding to any individual miRNA.

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   5 Working days

Packaging

   Lyophilized

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Store Clear-MiR™ miRNA inhibitors as a dry pellet at -20 °C (or preferably -70 °C) in a non-frost free freezer until ready to use. Once resuspended in RNase-free buffer, store at -70 °C and avoid contact with RNases. Clear-MiR™ miRNA inhibitors should be resuspended to a convenient stock concentration (20 to 50 µM) and stored in small aliquots to avoid multiple freeze thaw cycles. When stored under these conditions and using good RNase-free technique, they typically remain stable for 6 months or more. The solution can be freeze-thawed up to 5 times. For long-term storage, Clear-MiR™ miRNA inhibitors should be dried.

References: Krüztfeldt et al., Nucl. Acids Res., 2007, 35, p. 2885 - 2892
                     Krüztfeldt et al., Nature, 2005, 438, p. 685 - 689

Clear-MiR™ miRNA inhibitors are chemically modified antisense RNA oligonucleotides optimized to specifically target miRNA molecules in cells.
Use of Clear-MiR™ miRNA inhibitors enables miRNA functional analysis for down-regulation of miRNA activity (loss-of-function mutants). These optimized miRNA Inhibitors represents essential tools to confirm or determine miRNA function.

Key features of the Clear-MiR™ miRNA inhibitors

• Sequence-specific antisense inhibition of miRNA
• Resistance to nucleases
• Tight regulation of miRNA cellular levels
• Inhibition of miRNAs both in vitro and in vivo
• Available for more than 20 species and more than 3500 miRNA sequences
• Minimal cytotoxicity
• Easily transfected with standard methods
• Increased biological stability due to proprietary formulation

Clear-MiR™ oligos are available for all known miRNAs in invertebrates, vertebrates and plants, as registered and annotated in the miRNA Registry at The Wellcome Trust Sanger Institute. Clear-MiR™ oligos are available with different labels and can be linked to cholesterol for increase cellular uptake. On request, peptides can also be covalently linked to Clear-MiR™ oligos.


How to order your Clear-MiR™ (Plus)?

Send your order (with your P.O. number, delivery and invoice addresses) by e-mail to order@eurogentec.com mentioning :

• Accession Number of the corresponding miRNA
• Your choice between Clear-MiR™ miRNA inhibitor and Clear-MiR™ Plus miRNA inhibitor.
  We will design and synthesize the corresponding Clear-MiR™ (Plus) miRNA inhibitor.

Legal notices

   For Research Use only

Leaflets

• Clear-MiR™ miRNA inhibitors brochure 

 

 

Online Order not available at that time for Clear-MiR™ miRNA inhibitors.

• Online
  You can place your order online. Login to access our web ordering interface.

• By e-mail: order@eurogentec.com

• By fax : +32 4 264 07 88

• By mail
  Eurogentec S.A.
  LIEGE Science Park
  Rue du Bois Saint Jean 5
  4102 Seraing
  BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

• Clear-MiR™ Plus miRNA Inhibitors