How does gene immunisation work

Plasmids harboring genes to be expressed are transferred directly to the tissues of animal using needle injection or particle bombardment. These plasmid vectors, containing all elements required for expression in higher organisms then allow the in situ expression of antigenic proteins and the immune response is induced. From this starting point, many applications can be envisioned relating either to vaccine design and development, basic research in immunology or polyclonal antibody production against your protein.

Just send us

Your plasmid expression vector and we will send you the corresponding serum at the end of each month. The hosts available for this procedure are rabbits and mice.

Description	EUR
Rabbit or DNA immunisation	POR

Immunization procedure

• In order to improve DNA uptake, we induce a cycle of muscle degeneration and regeneration in the animal
• The DNA is injected into the regenerating muscle. We need at least 200 µg of vector per mouse (minimum 4 mice)
• Blood sampling is performed 4 weeks later and sera are sent to you for testing
• You receive 10–50 µl serum per mouse (300 µl for the final bleed) or 20 ml serum per rabbit (50 ml for the final bleed)
• You decide to continue the procedure or to stop at the end of each month according to your needs. If you continue, additional bleeds and boosts can be performed
• A modification of this protocol is always possible. Please contact us

Please provide all of the following information so that we may begin to process your order

 

				• Peptide sequence for anti-peptide programmes • Purchase order number from your institute • Product references and prices • Other important production information
				Delivery information • Name • Shipping Address • Telephone • Email	Invoicing information • Name • Shipping Address • Telephone • Email

• Online
  You can place your order online. Login to access our web ordering interface.

• By e-mail: order@eurogentec.com

• By fax : +32 4 264 07 88

• By mail
  Eurogentec S.A.
  LIEGE Science Park
  Rue du Bois Saint Jean 5
  4102 Seraing
  BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.