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Custom siRNA Online Order Request Design siRNA Design

The easiest and most efficient way to achieve RNAi is to use synthetic small interfering RNAs (siRNA). Standard siRNAs are duplexes of short mixed oligonucleotides. These are typically made of 19 RNA nucleotides with symmetric 2 nucleotides (usually DNA bases) 3’ overhangs.

Name	Synthesis scale	Purification	Reference	EUR
Duplex siRNA (2 complementary strands)	40 nmol	SePOP	OR-0030-NPSET2004	250.00
Duplex siRNA (2 complementary strands)	0.2 µmol	SePOP	OR-0030-NPSET202	333.00
Duplex siRNA (2 complementary strands)	1.0 µmol	SePOP	OR-0030-NPSET210	367.00
Duplex siRNA (2 complementary strands)	40 nmol	HPLC	OR-0030-HPSET2004	299.30
Duplex siRNA (2 complementary strands)	0.2 µmol	HPLC	OR-0030-HPSET202	391.70
Duplex siRNA (2 complementary strands)	1.0 µmol	HPLC	OR-0030-HPSET210	506.10
Duplex siRNA (2 complementary strands)	40 nmol	PAGE	OR-0030-SET2004	299.30
Duplex siRNA (2 complementary strands)	0.2 µmol	PAGE	OR-0030-SET202	391.70
Duplex siRNA (2 complementary strands)	1.0 µmol	PAGE	OR-0030-SET210	506.10
Single strand siRNA	40 nmol	SePOP	OR-0030-NPMIX004	142.00
Single strand siRNA	0.2 µmol	SePOP	OR-0030-NPMIX02	175.00
Single strand siRNA	1.0 µmol	SePOP	OR-0030-NPMIX10	235.00
Single strand siRNA	0.2 µmol	HPLC	OR-0030-HPMIX02	215.30
Single strand siRNA	1.0 µmol	HPLC	OR-0030-HPMIX10	336.00
Single strand siRNA	0.2 µmol	PAGE	OR-0030-MIX02	215.30
Single strand siRNA	1.0 µmol	PAGE	OR-0030-MIX10	336.00

Standard siRNA are made of 19 RNA bases + 2 DNA bases 3' overhang. Additional bases could be charged according to RNA list price

Quality Control

MALDI-TOF Mass Spectrometry

Delivery times

5 Working days

Packaging

Annealed in solution or not annealed lyophilized

Shipping conditions

Dry ice (solution) or Room temperature (lyophilized)

Storage conditions

-20 °C to -70 °C
Store siRNA oligos as a dry pellet at -20°C (or preferably -70 °C) in a non-frost free freezer until ready to use. Once resuspended in RNase-free buffer, store at -70 °C and avoid contact with RNases. siRNA oligos should be resuspended to a convenient stock concentration (20 to 50 µM) and stored in small aliquots to avoid multiple freeze thaw cycles. When stored under these conditions and using good RNase-free technique, they typically remain stable for 6 months or more. The solution can be freeze-thawed up to 5 times. For long-term storage, siRNA oligos should be dried.

SePOP siRNA : High pure desalted siRNA
To allow screening and sequence assessment, Eurogentec has developed an high-throughput small scale synthesis platform for the production of desalted siRNA.

2 complementary siRNA strands (19 RNA bases plus 2 x 3’-DNA overhang bases)
40 nmol synthesis scale
Desalted and fully deprotected
1 OD guaranteed per strand
100 % MALDI-TOF Mass Spectrometry controlled
In order to ensure maximum stability and, if not annealed, all siRNAs are provided lyophilized in individual tubes.
Free annealing on request (shipped with free annealing buffer and RNase-free water).

In order to up-scale experiments with a validated sequence, Eurogentec is also proposing the two following production scales: 0.2 and 1 µmol.

PAGE siRNA : High quality and yields

2 complementary siRNA strands (19 RNA bases plus 2 x 3’-DNA overhang bases)
0.2 µmol or 1 µmol synthesis scales
Desalted, fully deprotected and PAGE purified (average purity > 95 %)
0.2 µmol synthesis scale, 10 OD guaranteed per strand (50 nmol of a 21 mer)
1 µmol synthesis scale, 15 OD guaranteed per strand (80 nmol of a 21 mer)
100 % MALDI-TOF Mass Spectrometry controlled
In order to ensure maximum stability and, if not annealed, all siRNAs are provided lyophilized in individual tubes.
Free annealing on request (shipped with free annealing buffer and RNase-free water).

Please note that each single siRNA strand can be ordered separately.

Legal notices

For Research Use only

Order Forms

Leaflets

Oligonucleotides Catalogue

Product citations

POTENTE M. et al., "11,12-Epoxyeicosatrienoic Acid-induced Inhibition of FOXO Factors Promotes Endothelial Proliferation by Down-Regulating p27^Kip1", Journal of Biological Chemistry, vol. 278, n° 32, p. 29619-29625, May 2003

TUROWSKI P. et al., "Functional cdc25C Dual-Specificity Phosphatase Is Required for S-Phase Entry in Human Cells", Molecular Biology of the Cell, vol. 14, Issue 7, p. 2984-2998, July 2003

AVEROUST J. et al., "Induction of CHOP Expression by Amino Acid Limitation Requires Both ATF4 Expression ATF2 Phosphorylation", Journal of Biological Chemistry, vol. 279, n° 7, p. 5288-5297, February 2004

LAZRAK M. et al., "The bHLH TAL-1/SCL regulates endothelial cell migration and morphogenesis", Journal of Cell Science, 117, 1161-1171, February 2004

DUTTON A. et al., "Expression of the cellular FLICE-inhibitory protein (c-FLIP) protects Hodgkin's lymphoma cells from autonomous Fas-mediated death", PNAS, vol. 101, n° 17, p. 6611-6616, April 2004

VOURET-CRAVIARI V. et al., "ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells", Journal of Cell Science, 117, p. 4559-4569, May 2004

YU L.-G. et al., "Protein Phosphatase 2A, a Negative Regulator of the ERK Signaling Pathway, Is Activated by Tyrosine Phosphorylation of Putative HLA Class II-associated Protein I (PHAPI)/pp32 in Response to the Antiproliferative Lectin, Jacalin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41377-41383, October 2004

STEWART S. et al., "Epstein–Barr virus-encoded LMP2A regulates viral and cellular gene expression by modulation of the NF-B transcription factor pathway", PNAS, vol. 101, n° 44, 15730-15735, November 2004

LAIGLE A. et al., "The reduction of P-Glycoprotein Expression by small interfering RNAs is improved in exponentially growing cells", Oligonucleotides, 14:191-198, 2004

WÜNSCHE W. et al., "The activity of siRNA in Mammalian Cells is related to the kinetics of siRNA-target recognition in vitro: Mechanistic Implications", Journal of Molecular Biology, vol. 345, 203-209, 2005

LOUIS K. et al., "Tumor Cell-mediated Induction of the Stromal Factor Stromelysin-3 Requires Heterotypic Cell Contact-dependent Activation of Specific Protein Kinase C Isoforms", Journal of Biological Chemistry, vol. 280, n° 2, p. 1272-1283, January 2005

To order easily your siRNA sequences you can either download the following document (siRNA Email Order Form) or directly order your siRNA through our ordering web interface in the siRNA module.