Bacteria in a convenient format
High-efficiency competent E. coli are supplied in a 96-tube tray. This convenient format gives you the freedom to perform from 1 to 96 transformations at a time, without thawing more tubes of cells than needed. You can remove individual tubes by cutting cap strips. Thus, this format eliminates the reduced efficiencies that occur as a result of repeated freezing and thawing and makes possible high-throughput transformation.
Strain
The strain is convenient for use in cloning, plasmid preparation and library construction. It is endonuclease and recombination minus. It allows lacZa complementation for blue/white screening.
DG1: mcrA D(mrr-hsdRMS-mcrBC, modification-, restriction-) f80lacZDM15 DlacX74 recA1 araD139 D(ara-leu)7697 galU galK rpsL endA1 nupG
Efficiency
All the tests are performed with 10 ng DNA of a 6 kb plasmid (medium copy number: pBR322 replication).
Our bacteria allow high yields of any cloned DNA, including large BAC, plasmid, methylated or unmethylated inserts.
- Chemically-competent: 109 cfu/µg DNA (uncoloured tubes)