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Research Diagnostic Therapeutic

DG1 competent Bacteria in a convenient format

High-efficiency competent E. coli are supplied in a 96-tube tray. This convenient format gives you the freedom to perform from 1 to 96 transformations at a time, without thawing more tubes of cells than needed. You can remove individual tubes by cutting cap strips. Thus, this format eliminates the reduced efficiencies that occur as a result of repeated freezing and thawing and makes possible high-throughput transformation.

Name	Quantity	Reference	EUR
DG1 chemically-competent cells	24 x 50 µl	GE-DG1C-24	168.00
DG1 chemically-competent cells	48 x 50 µl	GE-DG1C-48	288.00
DG1 chemically-competent cells	96 x 50 µl	GE-DG1C-96	401.00
DG1 electro-competent cells	24 x 50 µl	GE-DG1E-24	240.00
DG1 electro-competent cells	48 x 50 µl	GE-DG1E-48	456.00
DG1 electro-competent cells	96 x 50 µl	GE-DG1E-96	864.00

Delivery times

2 working days

Shipping conditions

Dry ice

Storage conditions

-80°C

Bacteria in a convenient format

High-efficiency competent E. coli are supplied in a 96-tube tray. This convenient format gives you the freedom to perform from 1 to 96 transformations at a time, without thawing more tubes of cells than needed. You can remove individual tubes by cutting cap strips. Thus, this format eliminates the reduced efficiencies that occur as a result of repeated freezing and thawing and makes possible high-throughput transformation.

Strain

The strain is convenient for use in cloning, plasmid preparation and library construction. It is endonuclease and recombination minus. It allows lacZa complementation for blue/white screening.

DG1: mcrA D(mrr-hsdRMS-mcrBC, modification-, restriction-) f80lacZDM15 DlacX74 recA1 araD139 D(ara-leu)7697 galU galK rpsL endA1 nupG

Efficiency

All the tests are performed with 10 ng DNA of a 6 kb plasmid (medium copy number: pBR322 replication).

Chemically-competent: 108 cfu/µg DNA (uncoloured tubes)
Electro-competent: 109 cfu/µg DNA (green tubes)

Thus, our bacteria allow high yields of any cloned DNA, including large BAC, plasmid, methylated or unmethylated inserts.