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dNTPs/dUTP Mix

Life Science Molecular Diagnostics GMP BioManufacturing
dNTPs mixes are enzymatically produced from nucleotide monophosphates by a process of phosphorylation. This process uses highly specific enzymatic systems, which eliminate impurities and PCR inhibitors such as modified nucleotides and pyrophosphates. dNTPs are purified with preparative HPLC and are at least 99 % pure.

> A pre-mixed solution in water of 5 mM of each 2’-deoxy-adenosine-5’-triphosphate, 2’-deoxy-cytidine-5’triphosphate, 2’-deoxy-guanosine-5’-triphosphate and 10 mM 2’-deoxy-uridine-5’-triphosphate.


Name Quantity Reference EUR
dNTPs/dUTP Mix 1250 µl NU-0040-01 66.90


Quality Control


dNTPs are tested for the absence of DNase, RNase, protease and nicking activity. Each batch is validated rigorously for purity by HPLC, and tested for performance in a wide range of PCR templates, including a long-distance 20 kb functional test.

Delivery times

   2 working days

Shipping conditions

   -20°C

Storage conditions

   -20°C

A pre-mixed solution in water of 5 mM of each 2’-deoxy-adenosine-5’-triphosphate, 2’-deoxy-cytidine-5’triphosphate, 2’-deoxy-guanosine-5’-triphosphate and 10 mM 2’-deoxy-uridine-5’-triphosphate.

Application

   For direct use with the Uracil-DNA glycosylase (UNG) for the carry-over prevention technique (see Real-Time qPCR chapter).

PCR use

   Use 2 µl per 50 µl PCR for a final concentration of 200 µM each dATP, dCTP, dGTP, and 400 µM dUTP.

How to order online

   Online ordering is not available for this product


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    BELGIUM

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