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Research Diagnostic Therapeutic

HotGoldStar is a recombinant Hotstart activated-Taq DNA polymerase.

It is purified from an E. coli strain, which carries a Thermus species DNA polymerase overproducing plasmid.
It is a modified Taq polymerase, which completely lacks any activity below 74 °C that avoids non-specific priming at low temperature. HotGoldStar requires a thermal activation of 10 minutes at 95 °C to reach maximal initial activity. During the PCR the rest of its activity is released. It is heat-degraded in a much lower rate as commonly used Taq DNA polymerase.
Hotstart activity
No primer dimers
No non-specific products
TA cloning

Name Concentration Quantity Reference EUR

HotGoldStar DNA polymerase (hotstart) 5 U/µl 100 units ME-0073-01 75.00

HotGoldStar DNA polymerase (hotstart) 5 U/µl 500 units ME-0073-05 298.90

HotGoldStar DNA polymerase (hotstart) 5 U/µl 5000 units ME-0073-1ML 2988.00

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Quality Control

Each lot of HotGoldStar is tested extensively for the absence of nicking and priming activities, exonucleases and non-specific endonucleases.

Delivery times
   2 working days

Shipping conditions
   Room Temperature

Storage conditions
   -20°C

Hotstart activity
No primer dimers
No non-specific products
TA cloning

HotGoldStar is a recombinant Taq DNA polymerase isolated from extremely thermostable clones. It is purified from an E. coli strain, which carries a Thermus species DNA polymerase overproducing plasmid. It is a modified Taq polymerase, which completely lacks any activity below 74 °C that avoids non-specific priming at low temperature. HotGoldStar requires a thermal activation of 10 minutes at 95 °C to reach maximal initial activity. During the PCR the rest of its activity is released. It is heat-degraded in a much lower rate as commonly used Taq DNA polymerase.

Activity

The enzyme has a 5’ ’ 3’ polymerization-dependent exonuclease replacement activity but lacks a 3’ ’ 5’ exonuclease proofreading activity. The enzyme has the “extendase” activity allowing TA cloning.

Application

DNA fragments as long as 2 kb can be efficiently amplified. Thanks to its hotstart activity the HotGoldStar DNA polymerase will provide efficient amplification of specific products without amplifying non-specific products or primer dimers. This Taq DNA polymerase is optimal for Real-Time qPCR.