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Research Diagnostic Therapeutic

HotGoldStar Mix kit contains all PCR reaction componants except the primers and the template. It's a ready-to-use in 2x concentration. The kit contains sufficient reagents for 200 PCR reactions of 50 µl and an additional tube of MgCl2 to adjust the concentration if required.

2x MasterMixes
HotStart activatedTaq DNA polymerase (HotGoldStar DNA polymerase)
dNTP 200 µM (final concentration)
MgCl2 1.5 mM (final concentration)
2x buffer

Name Quantity Reference EUR

HotGoldStar Mix 200 RXNs PK-0073-02 168.90

Quality Control

Each lot is tested for activity in PCR and a specific PCR product will be amplified after 35 cycles. 10 % of the PCR products are visualized on a 1.5 % agarose gel. The PCR is performed in duplex, using serial dilutions of 100 ng, 10 ng, 0.1 ng DNA per 50 µl PCR.

Delivery times

2 working days

Shipping conditions

-20 °C

Storage conditions

12 months at -20 °C
3 months at 4 °C

HotStart activatedTaq DNA polymerase (HotGoldStar DNA polymerase)
dNTP 200 µM (final concentration)
MgCl2 1.5 mM (final concentration)
2x buffer

Easy-to-use

2x MasterMix containing Taq DNA polymerase, dNTPs, MgCl2, buffer
Extra tube of 25 mM MgCl2
Just add primers and DNA

Reproducibility

By handling watery solutions instead of pipetting 50 % glycerol Taq DNA polymerase solution, pipetting mistakes will be avoided and more reproducible results will be obtained.