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A human genome microarray made of sense-strand polynucleotide probes spotted onto a proprietary chemical layer coated on top of a (25 mm x 75 mm) standard-format microarray glass slide. Each probe is spotted onto the MicroArray in a highly consistent manner using a proprietary, non-contact spotting technology adapted for microarray manufacturing

Over 30,000 Genes Give You a Wealth of Information

30968 human genome probes and 1082 experimental control probes
Highly sensitive 60-mer sense-strand polynucleotide probes (25mm x 75mm) spotted on standard-format glass slide
Latest information of human genome content from public domains is used to design the probes that monitor the expression level of corresponding human protein-coding genes
Each probe is spotted onto the MicroArray in a highly consistent manner using an innovative non-contact spotting technology adapted for microarray manufacturing.

Customer Friendly Packaging

100% satisfaction & replacement guarantee
Probe annotation & scanner files
Comprehensive User Manual
High-performance hybridization buffer
Pre-hybridization chamber included

Name	Quantity	Reference	EUR
Human Whole Genome MicroArray	10	AR-HUMA-01	990.00

Quality Control

The Human Whole Genome Arrays are certified to generate a high quality data.
All printed slides are previously selected for coating uniformity and low intrinsic background.
The MicroArrays are visually inspected for spotting quality (doughnuts), uniformity, integrity and for perfect alignment of spots and grids.
From each batch of printed MicroArray, three are randomly chosen for further quality control. We use the Terminal Transferase reaction to assess the amount of DNA covalently attached to the slides. Finally, we perform a real hybridization using labeled cDNA obtained from heat-choc cultured cells.

Delivery times

5 WD

Packaging

The MicroArrays are shipped in a light-tight box and they can be stored at room temperature for the minimum o 3 months without loss of signal intensities.
The identity of each spot on the MicroArray is provided in a gene list as an Excel™ file to provide a road map for accurate data analysis.
The package includes comprehensive protocols for RNA extraction, cDNA synthesis, and labeling and hybridization conditions that give reproducible and, high-quality hybridization results.

Shipping conditions

Room temperature

Storage conditions

Room temperature

Bioinformatics Contents

Each 60-mer oligonucleotide probe is designed to hybridize to a specific target gene described in the latest public domain contents, such as Unigene, Cancer Genome Anatomy Project (CGAP), BioCarta, Kyoto Encyclopedia of Gene and Genome (KEGG), and validated by the Human Genome Sequencing Project.
The most well-known and studied target genes are the first priority of probe selection.
1082 performance monitoring control probes for hybridization, sample quality, and labeling reactions.
Also included are the SpotReport® Oligo Array Validation System, and Alien® Oligo Array Validation System by Stratagene, and ArrayControl™ Sense Oligo Spots by Ambion.

Table 1: Human Genome Content
*Human Genome MicroArray is guaranteed to print 95% or more of the total probe content.

Human Genome MicroArray Control Features

Grid Alignment Mark (GAM) (Probe ID Number: PH_c_0000001):

GAM control probe is a Cy3 labeled 60-mer oligonucleotide probe designed from an alien sequence that does not cross-hybridize with human targets. More than 90 GAM features are evenly distributed across the arrayed area, and these features can be excited by a 532 nm laser without hybridization. GAM probes can serve as orientation or grid-alignment landmarks. Figure 2 illustrates this.

Orientation Grid Alignment Mark (OGAM) (Probe ID Number: PH_c_0000089):

OGAM is a Cy3/Cy5 labeled 60-mer oligonucleotide probe designed from an alien sequence that does not cross-hybridize with human targets. It is located on top of the upper left corner of the print region and serves as an orientation landmark for template registration, as different scanners may have different image orientation output formats.

Figure 2: Human Genome MicroArray OGAM and CGAM Positions

Corner Grid Alignment Marks (CGAMs) (Probe ID Number: PH_c_0000072):

CGAMs are mixtures of Cy3/Cy5 labeled 60-mer oligo designed from an alien sequence that does not cross-hybridize with human targets. CGAMs are located at the four corners of the image and serve as the orientation landmarks for auto or manual grid alignment under Cy3 (532 nm) or Cy5 (635 nm) scanning channel. Figure 2, below, illustrates this.

Intrinsic Hybridization Controls (IHCs) (Probe ID Number: PH_c_0000025):

IHCs contain mixtures of probes from 58 robustly expressed house-keeping genes that serve as built-in positive controls. The probe mixture is dispensed at a fixed concentration, and more than 95 IHC features are evenly distributed across the arrayed area. The hybridization signal intensity of IHCs gives the indication of overall quality of sample processing and hybridization efficiency.

Intrinsic Hybridization Ladder (IHL) (Probe ID Numbers: PH_c_0000026 to PH_c_0000033):

The IHL probes are dispensed in serial dilutions of IHC controls. The data extracted from IHL after target hybridization can be used to construct a hybridization intensity response curve depending on different dilutions of the probe mixture, which will be able to serve as an indicator to monitor the overall consistency of sample processing and hybridization stringency.
Table 3, below, provides the ID number of the IHL controls and detailed information about their content.

Table 3: Human Genome MicroArray IHL Control Probe Data Intrinsic

Target Quality Control (ITQC) (Probe ID Nu: PH_c_0000038 to PH_c_0000065):

Four probes per gene were selected from seven consistently expressed house-keeping genes. For each gene, one probe is designed from regions of 300-600 bp, 900-1200 bp, 1500-1800 bp, and 2100-2400 bp, respectively, from each 3’ end of the transcript. Tables 4 and 5, below, provide ITQC Probe information.

Table 4: Human Genome MicroArray ITQC Gene Numbers and Names

Table 5: Human Genome MicroArray ITQC Probes Negative Control/Extrinsic

Target Quality Control (ETQC) (Probe ID : PH_c_0000066 to PH_c_0000071):

ETQC probes are alien probe sequences that do not cross-hybridize with human genomic probes on Human Genome MicroArray . The targets to these probes can be prepared separately as RNA containing a poly-A tail and spiked into the experimental RNA sample to monitor target preparation quality and determine the sensitivity of the hybridization reaction.

Ambion ArrayControl™ RNA SPIKE #1~8 (Probe ID : AM_c_0000015 ~ PH_c_0000022):

Additional information about Ambion ArrayControl RNA SPIKE #1~8 can be found at: www.ambion.com Sequence information of Array Control RNA SPIKE #1~8 from Ambion, Inc., has been licensed for use with the Human Genome MicroArray. Using the sequence information, eight 60-mer oligonucleotide probes are designed to compliment the respective RNA SPIKES. These probes are alien probes sequences that do not cross-hybridize with human genomic probes on the Human Genome MicroArray , and thereby provide a set of of negative controls, which can be used as spike-in positive controls to determine the sensitivity of the hybridization process. Table 11, below, lists these probe ID numbers and their corresponding names.

Legal notices

The use of this Array-related product in relation to the manufacture or use of nucleic acid arrays may be covered by the following patent owned by Oxford Gene Technology Limited ("OGT"): EP 0,373,203. The purchase of this product does not confer on the purchaser any rights or licences under any OGT patent. To enquire about a licence under OGT's patents, please contact licensing@ogt.co.uk.