New supramolecular organization
Complexation of DNA molecules with the ICAFectin™ 441 transfection reagent lead to the formation of novel discrete compact and mushroom like structures with a size in the 200 nm range. This leads to an optimal DNA delivery into the cells.
High transfection efficiency in almost any cells compared to existing products on the market
Select the next-generation reagent that has been used successfully to transfect cell types derived from diverse species and tissues, including:
- Cancer cell lines
- Adherent and suspension cells
- Established cell lines
- Primary cells
- Stem cells
Embryonic stem cells (R1)
Mesenchymal stem cells (BMC9
Primary Hepatocytes
Neurons
PC12 (neuronals-like cells)
COS-7 (Fibroblast-like cells)
Pictures: Fluorescent microscopy visualisation of cells transfected with 0.5 µg DNA encoding the fluorescent protein GFP using the ICAFectin™441 reagent. The transfected cells were observed 24 hours after transfection using a FITC filter to visualise GFP fluorescence (right) or by light microscopy (left) to visualise cell morphology.
Graphs: Luciferase activity 24 hours after transfection of cells with 0.5 µg DNA encoding luciferase complexed with ICAFectin™441 compared to that obtained with competitor reagents.
Easy-to-use procedure
ICAFectin™
441 Transfection Reagent is provided as a ready-to-use solution - Simply add the reagent to your diluted DNA, mix, incubate, and pipet the complexes onto the cells. Transfections can be performed in the presence of serum, eliminating the need to remove complexes from the cells.
Absence of cytotoxicity using ICAFectin™ 441
Cell viability analysis after transfection using ICAFectin™ 441 or competitors reagents.
Ideal for high-throughput applications
The simple and rapid reverse transfection protocol of ICAFectin™
441 makes it ideal for high-throughput transfections for transient protein expression and cDNA library screening. Transfection can be easily established for automated or robotic systems.