ICAFectin™ 442 Transfection Reagent is based on an innovative synthetic derivative of a natural compound, and is used to transfect siRNA for RNA interference applications into a wide range of cell types such as HeLa, HEK 293, HepG2, MCF-7, HUVEC, HaCaT cells and many others.
Easy handling
• The ICAFectin™ 442 / siRNA complex can be transfected in cells in the presence of serum.
• ICAFectin™ 442 is non-toxic, the removal of transfection complex is not needed.
• ICAFectin™ 442 / siRNA complex may be kept at RT during transfection.
High-throughput compatible
The simple and rapid reverse transfection protocol of ICAFectin™ 442 makes it ideal for high-throughput transfections for transient protein expression and cDNA library screening. Transfection can easily be established for automated or robotic systems.
Outstanding transfection efficiency
No off-target effect!
ICAFectin™ 442 leads to a high release of siRNA within the cell and allows to use low concentration of siRNA. Reducing the amount of siRNAs used for transfections down to 1-20 nM minimizes changes in gene expression in mammalian cultured cells (off-target effects).
The knockdown efficiency of ICAFectin™ 442 was compared to other products available on the market. ICAFectin™ 442 shows the highest efficiency with a variety of cell lines.
Gene silencing in rat primary hepatocytes
| | Lamin A/C silencing efficiency using ICAFectin™442 reagent or competitor reagents
|
 | |  |
In each condition, residual Lamin A/C expression was determined to be 8-10 % of the control cells. Interestingly, identical Lamin A/C expression was obtained in cells transfected with non-sliencing siRNA and in untransfected cells. This indicates that the transfection with ICAFectin™ 442 did not affect the endogenous Lamin A/C expression and is devoid of any “off-target effect” | | Anti-LaminA/C siRNA at 2 and 4 nM (18.75 and 37.5 ng/well) were formulated with ICAFectin™442 or commercial reagents. Results of real-time quantitative RT-PCR analysis of human Lamin A/C mRNA after transfection of HeLa cells showed that ICAFectin™442 reagent was more efficient than the top leader products on the market. |
| | | |
GFP silencing efficiency using ICAFectin™ 442
|
|
Fluorescence microscopy visualization of GFP silencing and siRNA internalization. The GFP-expressing human lung cancer H1299 cells were transfected with control siRNA (A,B) or 3’-rhodamine labeled anti-GFP siRNA (C-F). The siRNA molecules were formulated in the absence (“naked” siRNA in A,C,E) or in the presence of ICAFectin™ 442 reagent (B,D,F). The transfected H1299 cells were observed using a FITC filter to visualize GFP fluorescence (A-B) or a rhodamine filter to visualize siRNA internalization (E,F). |