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LC hybridization probes

Life Science Molecular Diagnostics GMP BioManufacturing
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LC hybridization probes, also called FRET probes, are labelled with a single fluorescent reporter. Two LC hybridization probes specific for two adjacent sequences in the target DNA are used for quantitative Real-Time PCR.

Name Synthesis scale Recommended Purification* IncludedPurification* Reference EUR
LC Hybridization Probe 3’ FAM 200 nmol N/A N/A PB-LC100-020 171.40
LC Hybridization Probe 5’ ROX 200 nmol N/A N/A PB-LC016-020 306.00
LC Hybridization Probe 3’ Cy5® 200 nmol N/A N/A PB-LC055-020 257.00
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry and Analytical HPLC

Delivery times

   15-39 bases: 12 Working days
                             40-80 bases: 15 Working days

Packaging

   Lyophilised

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Lyophilized DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

LC hybridization probes, also called FRET probes, are labelled with a single fluorescent reporter. Two LC hybridization probes specific for two adjacent sequences in the target DNA are used for quantitative Real-Time PCR. One probe is labelled with a donor fluorophore and the other labelled with an acceptor (or reporter) fluorophore. Both LC hybridization probes are designed to anneal between the upstream and downstream PCR primers. When the probes are not bound to the target sequence, the fluorescent signal from the reporter dye is not detected. However, when the probes hybridize to the target sequence during the PCR annealing step, the close proximity of the two fluorophores allows energy transfer from the donor to the acceptor dye, resulting in a fluorescent signal that is detected. The amount of signal is proportional to the amount of target sequence, and is measured in real time to allow quantification of the amount of target sequence.

LC hybridization probes are usually constructed with FAM as the 3’ donor fluorophore, and ROX or Cy® 5 as the 5’ acceptor fluorophore. A 3’ phosphate group is also added to prevent extension of the reporter probe by Taq DNA polymerase during the PCR cycles. All probes are HPLC purified and controlled by MALDI-TOF Mass Spectrometry. LC hybridization probes are provided lyophilized in individual tubes.

Legal notices

   For Research Use only

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  • By e-mail: order@eurogentec.com
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

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