Our Lithos qPCR MasterMix, 2 mM, will allow you to achieve reliable, consistent and accurate Real-Time qPCR results for SYBR® Green and probe detection.
The Lithos qPCR MasterMix, 2 mM contains enough reagents for up to 350 - 20 µl reactions using the hotstart enzyme, GoldStar® and has been validated on capillary Real-Time thermocyclers.
The kit contains a 2x buffer dNTP/dUTP mix, GoldStar® and 2 mM MgCl2 tubes. Separate tube of SYBR® Green I and MgCl2 tubes are included in the kit.
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FOR RESEARCH USE ONLY
NOTICE TO PURCHASER: LIMITED LICENSE
A license under U.S. Patents 4,683,202, 4,683,195 and 4,965,188 or their foreign counterparts, owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd (Roche”), has an up-front fee component and a running-royalty component. The purchase price of this product includes limited, non-transferrable rights under the running-royalty component to use only this amount of the product to practice the Polymerase Chain Reaction (“PCR”) and related processes described in said patents solely for the research and development activities of the purchaser when this product is used in conjunction with a thermal cycler whose use is covered by the up-front fee component. Rights to the up-front fee component must be obtained by the end user in order to have a complete license. These rights under the up-front fee component may be purchased from Applied Biosystems or obtained by purchasing an Authorized Thermal Cycler. No right to perform or offer commercial services of any kind using PCR, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is hereby granted by implication or estoppel. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Director of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404 or the Licensing Department at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501.
Licensed dsDNA-Binding Dye Research Kits - Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
The use of certain fluorogenic probes in 5' nuclease assays may be covered by U.S. Patents 5,210,015 and 5,487,972, owned by Roche Molecular Systems, Inc, and by U.S. Patent 5,538,848, owned by The Perkin-Elmer Corporation. The purchase of Eurogentec’s product does not provide a license to use this patented technology. A license must be obtained by contacting the Director of Licensing Applied Biosystems, 850 Lincoln Centre Drive, Foster City, CA 94404 or the Licensing Department at Roche Molecular Systems Inc., 1145 Atlantic Avenue, Almeda, CA 94501.
Cyclic-substituted unsymmetrical cyanine dyes are covered by U.S. Patents 5,436,134 and 5,658,751 and licensed to Eurogentec S.A by Molecular Probes, Inc in the direct research field.
Use of UDG employs U.S. Patents 5,035,936, 5,945,313, 5,683,896 and their foreign counterparts licensed to Eurogentec, S.A from Invitrogen Corporation.