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MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX w/ fluorescein

Research Diagnostic Therapeutic
MESA GREEN qPCR Mast... Order
When using a SYBR® Green I kit on an iCycler iQ®, iQ5 or My iQ®, fluorescein is needed to normalize the data correctly. This is why Eurogentec has developed a SYBR® Green I kit that is already containing fluorescein in a ready-to-use MasterMix. Alternatively a separate tube of fluorescein is available in case you would like to use fluorescein in combination of any of our No ROX SYBR® Green I kits.

The MESA GREEN qPCR MasterMix Plus for SYBR® Assay w/ fluorescein contains enough reagents for up to 300 / 600 / 2000- 50 µl reactions using our new powerful MeteorTaq hotstart polymerase and has been validated on iCycler iQ®, My iQ®, iQ5.


Name Quantity Reference EUR
MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX w/ fluorescein 7.5 ml 300 RT-SY2X-03+WOUFL 475.00
MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX w/ fluorescein 15 ml 600 RT-SY2X-06+WOUFL 850.00
MESA GREEN qPCR MasterMix Plus for SYBR® Assay No ROX w/ fluorescein 50 ml 2000 RT-SY2X-20+WOUFL 2550.00
50 µl reactions

Sample request

Delivery times

   2 WD if ordered before Thursday

Shipping conditions

   Dry ice

Storage conditions


For long term storage the MESA GREEN qPCR MasterMix should be stored at -15 °C to -25 °C in a constant temperature freezer. When stored under these conditions the reagents are stable for 1 year.
For short term storage the MESA GREEN qPCR MasterMix can be stored at 4 °C to 6 °C for 1 month.
Minimize exposure of the MESA GREEN qPCR MasterMix to direct light to avoid any loss of fluorescent signal intensity.  
Avoid repeated freeze-thaw cycles.


When using a SYBR® Green I kit on an iCycler iQ®, iQ5 or My iQ®, fluorescein is needed to normalize the data correctly. This is why Eurogentec has developed a SYBR® Green I kit that is already containing fluorescein in a ready-to-use MasterMix. Alternatively a separate tube of fluorescein is available in case you would like to use fluorescein in combination of any of our No ROX SYBR® Green I kits.Our new generation of SYBR® Green reagents the MESA GREEN qPCR MasterMix Plus for SYBR® Assay w/ fluorescein, will allow you to achieve reliable, consistent and accurate Real-Time qPCR results. Our new MESA GREEN MasterMix w/ fluorescein contains our newly designed hotstart enzyme, "MeteorTaq", that will ensure, together with its newly formulated buffer, maximum specificity and sensitivity for you Real-Time qPCR SYBR® Green assay. The MESA GREEN qPCR MasterMix Plus for SYBR® Assay w/ fluorescein contains enough reagents for up to 300 / 600 / 2000 - 50 µl reactions using the hotstart enzyme, MeteorTaq. The 2x buffer contains dNTP/dUTP mix (no UNG), MeteroTaq, MgCl2, SYBR® Green I, fluorescein and stabilizers. An extra tube of 50 mM MgCl2 is provided in the kit to optimize MgCl2 concentration if necessary.

Legal notices


FOR RESEARCH USE ONLY - NOTICE TO PURCHASER: LIMITED LICENSE - Licensed dsDNA-Binding Dye Research Kits - Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652, 5,994,056, 6,171,785, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser’s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Cyclic-substituted unsymmetrical cyanine dyes are covered by U.S. Patents 5,436,134 and 5,658,751 and licensed to Eurogentec S.A by Molecular Probes, Inc in the direct research field.

Leaflets

TDS

MSDS

Certificate of analysis


To obtain the Certificate of Analysis corresponding to your qPCR kit of interest please contact us via email and display the reference code of the qPCR kit and the batch number. Contact us

Product citations


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EBNER K. et al., "Molecular Detection and Quantitative Analysis of the Entire Spectrum of Human Adenoviruses by a Two-Reaction Real-Time PCR Assay", Journal of Clinical Microbiology, vol. 43, n° 7, p. 3049-3053, July 2005


Ishibashi Y. et al., "Regulation of sialyl-Lewis x epitope expression by TNF-alpha and EGF in an airway carcinoma cell line", Glycoconjugate Journal, 22(1-2):53-62, February 2005


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DECOUSSER J.-W. et al., "New Real-Time PCR Assay Using Locked Nucleic Acid Probes To Assess Prevalence of ParC Mutations in Fluoroquinolone-Susceptible Streptococcus pneumoniae Isolates from France", Antimicrobial Agents and Chemotherapy, p. 1594-1598, Vol. 50, No. 4, April 2006




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