There is often some confusion surrounding the issues of synthesis scale and minimum guaranteed yield in custom oligonucleotide manufacturing:
- The synthesis scale refers to the amount of raw material used to start the synthesis of oligonucleotides.
- The yield corresponds to the amout of final product recovered at the end of the synthesis and purification processes.
The length, the sequence, the type and number of modifications and a purification, strongly influence the amount of synthetized material and proportion of full length oligonucleotides.
Based on that, Eurogentec defined a minimum guaranteed yield in OD and nmoles for all product categories (see tables below). The applied minimum guaranteed yield is the minimum Guaranteed Yield in OD (Table 1) or minimum Guaranteed Yield in nmoles (Table 2), whichever is lower. In many cases the minimum guaranteed yield represent only a reference because the delivered quantities are significantly higher.
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