There is often some confusion surrounding the issues of synthesis scale and minimum guaranteed yield in custom oligonucleotide manufacturing:
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The synthesis scale refers to the amount of raw material used to start the synthesis of oligonucleotides.
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The yield corresponds to the amout of final product recovered at the end of the synthesis and purification processes.
The length, the sequence, the type and number of modifications and a purification, strongly influence the amount of synthetized material and proportion of full length oligonucleotides.
Based on that, Eurogentec defined a minimum guaranteed yield in OD and nmoles for all product categories (see tables below). The applied minimum guaranteed yield is the minimum Guaranteed Yield in OD (Table 1) or minimum Guaranteed Yield in nmoles (Table 2), whichever is lower. In many cases the minimum guaranteed yield represent only a reference because the delivered quantities are significantly higher.
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Tables 1 and 2: (1) Between 2 and 59 bases length single-modified oligonucleotides. Eurogentec does not offer minimum guaranteed yield for modified oligonucleotides greater than 60 bases. Post-synthesis modifications (not compatibile with SePOP and RP-Cartridge purification) may yield 50% less than the above stated values. Alower yield may result from poly-modifications and/or strong secondary structures ; (2) Double-Dye probes only result from the combination of a 5’ fluorescent dye and a 3’ quencher. Post-synthesis modifications may yield 50% less than the above stated values. (3) Except for oligonucleotides with GC-rich regions;* Only available for Double-Dye FAM-TAMRA 10 nmol; For more information, please contact our Oligo Centre at oligo@eurogentec.com or visit our website www.eurogentec.com.