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MiRmaid miRNA precursors qRT-PCR control sets

Research Diagnostic Therapeutic
The MiRmaid miRNA precursors qRT-PCR control sets were developed and optimized to be used with the MiRmaid miRNA precursors qRT-PCR indiviual sets. The intended use of the MiRmaid miRNA precursors qRT-PCR primer sets is the amplification and detection of the human precursor microRNAs (pre-miRNAs) by performing a reverse transcription (RT) followed by a Real-Time qPCR using SYBR® Green I assay.
Click here to learn more about the MiRmaid miRNA precursors qRT-PCR individual sets.


Name Quantity Reference EUR
MiRmaid miRNA precursor qRT-PCR control set – U6 U6 RT primer for 400 RT U6 qPCR primer mix for 200 qPCR reactions RT-OLMI-U6 140.00
MiRmaid miRNA precursor qRT-PCR control set – 5S 5S RT primer for 400 RT 5S qPCR primer mix for 200 qPCR reactions RT-OLMI-5S 140.00
MiRmaid miRNA precursor qRT-PCR control set – HMBS HMBS RT primer for 400 RT HMBS qPCR primer mix for 200 qPCR reactions RT-OLMI-HMBS 140.00
MiRmaid miRNA precursor qRT-PCR control set – TBP TBP RT primer for 400 RT TBP qPCR primer mix for 200 qPCR reactions RT-OLMI-TBP 140.00
25 µl qPCR reactions

Packaging


The MiRmaid miRNA precursors qRT-PCR control sets contain:
- MiRmaid RT primer (green cap): one tube of 7 µl MiRmaid RT primer at 50 µM each. Sufficient to perform 400 RT reactions (for 2 X 96 qPCR reactions)
- MiRmaid qPCR primer set containing 896 µl of the MiRmaid Reverse and Forward primers at 2.5 µM.

Shipping conditions

   Dry ice

Storage conditions


The MiRmaid miRNA precursors qRT-PCR control sets are shipped on dry ice.
Upon receipt, the MiRmaid RT primer and the MiRmaid qPCR primer mix should be stored at -20 °C (-18 °C to –24 °C) in a constant temperature freezer to maintain stability. Repeated freeze-thaw cycles (more than 5) should be avoided.
When stored under these conditions the reagents are stable for at least 1 year.

MiRmaid illustration


The MiRmaid miRNA precursors qRT-PCR control sets were developed and optimized to be used with the MiRmaid miRNA precursors qRT-PCR indiviual sets. The intended use of the MiRmaid miRNA precursors qRT-PCR primer sets is the amplification and detection of the human precursor microRNAs (pre-miRNAs) by performing a reverse transcription (RT) followed by a Real-Time qPCR using SYBR® Green I assay. 

Click here to learn more about the MiRmaid miRNA precursors qRT-PCR indiviual sets.


The MiRmaid miRNA precursors qRT-PCR control sets contain sufficient MiRmaid RT primer to perform 400 RT (Reverse Transcriptase) reactions. The amount of cDNA produced after 1 RT reaction should allow performing 96 qPCR reactions. The MiRmaid qPCR primer mixes contain specific Reverse and Forward primers. These Forward and Reverse primer mixes are sufficient to performed at least 200 Real-Time qPCR reactions.
MiRmaid product picture

Four different controls can be ordered separatly. U6 and 5S are small size RNAs comparable to the size of pre-miRNAs. By amplifying the U6 and 5S positive controls by Real-Time qPCR you will be able to check that the qPCR reaction have been performed correctly and that any small RNAs have been well extracted, reverse transcribed to cDNA and then have been amplified correctly.
Pre-miRNAs are very low expressed RNAs, however 6U and 5S positive controls are highly expressed RNAs, this is why we also recommend to use 2 others low expressed positive controls, HMBS and TBP. These 2 positive controls are coming out at Ct values that are more or less comparable to the Ct values expected when amplifying precursor miRNAs.

How to order by e-mail


Send your orders by email. To get the template ordering form, please contact our Customer Help Desk or send an e-mail to info@eurogentec.com.
Do not hesitate to contact us to set up the best ordering method for you.

How to order by fax


It is possible to order your product by fax; please check the fax numbers at the bottom of each page of our catalogue or below. This ordering method requires more manpower than the previously mentioned ones any specifications in our system. Moreover we are applying a double check to avoid any mistakes. The treatment of these orders requires one additional working day to the standard delivery times. For fax orders an additional fee of 2.50 eur per product will be charged. However, we do not recommend fax ordering, because these orders have to be processed manually and are therefore errorprone.

How to order by mail


It is possible to order your MirMaid products by mail; please use the following adress:

Do not hesitate to contact us for any additional information or comments on these ordering tools.


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  • By fax : +32 4 264 07 88
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    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

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