While chemical modification of siRNA is usually not required, certain modifications can sometimes be useful e.g. to increase stability or cellular uptake
| Name |
Synthesis scale |
Purification |
Reference |
EUR |
|
| 5' Phosphate |
0.2 µmol |
RP-HPLC |
OR-0211-0502 |
46.40 |
|
| 5' Amine-C6 |
0.2 µmol |
RP-HPLC |
OR-0222-0502 |
38.00 |
|
| 3' Amine-C6 |
0.2 µmol |
PAGE |
OR-0222-0302 |
38.00 |
|
| 3' TEG-Cholesteryl |
0.2 µmol |
PAGE |
OR-0311-0302 |
82.20 |
|
| 5' 6 FAM |
0.2 µmol |
RP-HPLC |
OR-0353-02 |
92.70 |
|
| 3' 6 FAM |
0.2 µmol |
PAGE |
OR-0353-0302 |
154.50 |
|
| 5' HEX |
0.2 µmol |
RP-HPLC |
OR-0351-02 |
92.70 |
|
| 5' TET |
0.2 µmol |
RP-HPLC |
OR-0352-02 |
92.70 |
|
| 5' TAMRA |
0.2 µmol |
RP-HPLC |
OR-0355-02 |
129.80 |
|
| 3' TAMRA |
0.2 µmol |
PAGE |
OR-0357-02 |
103.00 |
|
| 5' Cy® 3 |
0.2 µmol |
RP-HPLC |
OR-CY3-02 |
108.20 |
|
| 3' Cy® 3 |
0.2 µmol |
PAGE |
OR-CY3-0302 |
190.60 |
|
| 5' Cy5® |
0.2 µmol |
RP-HPLC |
OR-CY5-02 |
108.20 |
|
| 3' Cy5® |
0.2 µmol |
PAGE |
OR-CY5-0302 |
190.60 |
|
| 5' Alexa 488 |
0.2 µmol |
RP-HPLC |
OR-0298-AL48802 |
350.00 |
|
| 5' Alexa 546 |
0.2 µmol |
RP-HPLC |
OR-0298-AL54602 |
350.00 |
|
| 5' Alexa647 |
0.2 µmol |
RP-HPLC |
OR-0298-AL64702 |
350.00 |
|
| 5' ATTO 488 |
0.2 µmol |
RP-HPLC |
OR-601-488ATT02 |
175.00 |
|
| 5' ATTO 550 |
0.2 µmol |
RP-HPLC |
OR-601-550ATT02 |
175.00 |
|
| 5' ATTO 655 |
0.2 µmol |
RP-HPLC |
OR-601-655ATT02 |
175.00 |
|
Price per modification
For other modifications, please inquireQuality Control MALDI-TOF Mass Spectrometry Delivery times 7-8 Working days Packaging Annealed in solution or not annealed lyophilized Shipping conditions Dry ice (solution) or Room temperature (lyophilized) Storage conditions -20 °C to -70 °C Store siRNA oligos as a dry pellet at -20°C (or preferably -70°C) in a non-frost free freezer until ready to use. Once resuspended in RNase-free buffer, store at -70°C and avoid contact with RNases. siRNA oligos should be resuspended to a convenient stock concentration (20 to 50 µM) and stored in small aliquots to avoid multiple freeze thaw cycles. When stored under these conditions and using good RNase-free technique, they typically remain stable for 6 months or more. The solution can be freeze-thawed up to 5 times. For long-term storage, siRNA oligos should be dried.
While chemical modification of siRNA is usually not required, certain modifications can sometimes be useful e.g. to increase stability or cellular uptake. The antisense strand must either have a free 5’-OH (by default) or 5’-phosphate terminus. 5’-end modification of the sense strand RNA does not alter the efficiency of silencing. Various fluorescent dyes can be coupled to the 5’-end of the sense strand oligo to track transfection efficiency of the corresponding duplex. Finally, modifying siRNA with cholesterol is used to facilitate tissue / cellular uptake. Modifications, such as 2’O-methyl RNA, phosphorothioate bonds, LNA® and a variety of other options are also available. As a consequence, Eurogentec is continuously extending its list of modifications (see table below). If the one you need is not included, please inquire. All modified siRNAs are based on the 0.2 µmol synthesis scale and extensively purified using the most appropriate purification method. Other scales, overhang chemistry and base contents are available on request. Legal notices For Research Use only
LeafletsProduct citationsPOTENTE M. et al., "11,12-Epoxyeicosatrienoic Acid-induced Inhibition of FOXO Factors Promotes Endothelial Proliferation by Down-Regulating p27Kip1", Journal of Biological Chemistry, vol. 278, n° 32, p. 29619-29625, May 2003 TUROWSKI P. et al., "Functional cdc25C Dual-Specificity Phosphatase Is Required for S-Phase Entry in Human Cells", Molecular Biology of the Cell, vol. 14, Issue 7, p. 2984-2998, July 2003 AVEROUST J. et al., "Induction of CHOP Expression by Amino Acid Limitation Requires Both ATF4 Expression ATF2 Phosphorylation", Journal of Biological Chemistry, vol. 279, n° 7, p. 5288-5297, February 2004 LAZRAK M. et al., "The bHLH TAL-1/SCL regulates endothelial cell migration and morphogenesis", Journal of Cell Science, 117, 1161-1171, February 2004 DUTTON A. et al., "Expression of the cellular FLICE-inhibitory protein (c-FLIP) protects Hodgkin's lymphoma cells from autonomous Fas-mediated death", PNAS, vol. 101, n° 17, p. 6611-6616, April 2004 VOURET-CRAVIARI V. et al., "ILK is required for the assembly of matrix-forming adhesions and capillary morphogenesis in endothelial cells", Journal of Cell Science, 117, p. 4559-4569, May 2004 YU L.-G. et al., "Protein Phosphatase 2A, a Negative Regulator of the ERK Signaling Pathway, Is Activated by Tyrosine Phosphorylation of Putative HLA Class II-associated Protein I (PHAPI)/pp32 in Response to the Antiproliferative Lectin, Jacalin", Journal of Biological Chemistry, vol. 279, n° 40, p. 41377-41383, October 2004 STEWART S. et al., "Epstein–Barr virus-encoded LMP2A regulates viral and cellular gene expression by modulation of the NF-B transcription factor pathway", PNAS, vol. 101, n° 44, 15730-15735, November 2004 LAIGLE A. et al., "The reduction of P-Glycoprotein Expression by small interfering RNAs is improved in exponentially growing cells", Oligonucleotides, 14:191-198, 2004 WÜNSCHE W. et al., "The activity of siRNA in Mammalian Cells is related to the kinetics of siRNA-target recognition in vitro: Mechanistic Implications", Journal of Molecular Biology, vol. 345, 203-209, 2005 LOUIS K. et al., "Tumor Cell-mediated Induction of the Stromal Factor Stromelysin-3 Requires Heterotypic Cell Contact-dependent Activation of Specific Protein Kinase C Isoforms", Journal of Biological Chemistry, vol. 280, n° 2, p. 1272-1283, January 2005
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