To coordinate and monitor the large synthetic projects, Eurogentec has designed and implemented the necessary database infrastructure to support high-throughput synthetic efforts. This laboratory information management system assists in oligonucleotide ordering, oligonucleotide annotation, tracking, and oligonucleotide quality control. 

Chemical oligo synthesis: 

• 60 mers with 5’ C6-NH2 modification 
• Scale synthesis: 40 nmoles· 
• Purification: Sepop · 
• Quantity guaranty: 4 nanomoles
• Concentration normalized at 100 nM 
• Quality control: oligo analyzed by MS

Name		Reference	EUR
Oligonucleotide Synthesis and Quality Control		AR-LOLIG-60	Upon request

Quality Control

The following quality control metrics are applied to assess the quality and the quantity of the oligo.

1- Oligonucleotide synthesis are monitored by the trityl release following the first coupling and the last coupling. The step-wise repetitive yield is computed based on the total number of couplings.
2- Oligonucleotides with less than a 99 % coupling efficiency are rejected. After the final detritylation, the oligo is 5'-modified with a C6 amino-linker using standard phosphoramidite coupling chemistry. The oligonucleotides are subsequently deprotected, desalted, and the optical density of each amino-modified measured at 260 nm.
3- To monitor heterogeneity in oligonucleotide length, each oligonucleotide is subjected to control. The quality of the oligo’s will be controlled by the measurement of the molecular mass using MALDI-TOF Mass Spectrometry (MS). All the oligo showing a bad / poor MS profile will be re-synthesized at the expense of Eurogentec.

The sequence information of the designed probes is forwarded to our in-house oligo production team. 
In order to satisfy the demand of our customers, Eurogentec has established a high-throughput synthesis program capable for example of producing up to 500 5'-amino modified probes of 70 bases per day. 
To coordinate and monitor the large synthetic projects, Eurogentec has designed and implemented the necessary database infrastructure to support high-throughput synthetic efforts. This laboratory information management system assists in oligonucleotide ordering, oligonucleotide annotation, tracking, and oligonucleotide quality control. 
The amino-modified probes used for microarray fabrication are exhaustively analyzed to ensure probes are full-length and to guarantee the minimal yield of 4 nanomole per oligo. 

The following quality control metrics are applied to assess the quality and the quantity of the oligo:

1- Oligonucleotide syntheses are monitored by the trityl release following the first coupling and the last coupling. The step-wise repetitive yield is computed based on the total number of couplings.
 
2- Oligonucleotides with less than a 99% coupling efficiency are rejected. After the final detritylation, the oligo is 5'-modified with a C6 amino-linker using standard phosphoramidite coupling chemistry. The oligonucleotides are subsequently deprotected, desalted, and the optical density of each amino-modified measured at 260 nm.

3- To monitor heterogeneity in oligonucleotide length, each oligonucleotide is subjected to control. The quality of the oligo’s will be controlled by the measurement of the molecular mass using MALDI-TOF Mass Spectrometry (MS). All the oligo showing a bad / poor MS profile will be re-synthesized at the expense of Eurogentec.

• For specific quote and more information, please contact Dr Driss Talibi:
  
  dr.talibi@eurogentec.com
  Tel.: +32 4 372 74 39
  Mobile: +32 478 366 619
  Fax: +32 4 372 75 00

• Online
  You can place your order online. Login to access our web ordering interface.

• By e-mail: order@eurogentec.com

• By fax : +32 4 264 07 88

• By mail
  Eurogentec S.A.
  LIEGE Science Park
  Rue du Bois Saint Jean 5
  4102 Seraing
  BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.