Phosphate

Research Diagnostic Therapeutic
Phosphate Build & Order

The most current application of the 5’-PO4 modified oligonucleotide is the direct ligation to a vector without prior use of T4 polynucleotide kinase. 3’-PO4 and 5’-PO4 oligonucleotides may also be used for either further chemical oligonucleotide modification or as a substrate in DNA repair studies. This phosphorylation permits the labelling of large quantities and is more reproducible than enzymatic procedures.

Name Synthesis scale Recommended Purification* Required Purification* Reference EUR
5' Phosphate 40 nmol RP-HPLC - MD-PH001-05004 20.00
5' Phosphate 200 nmol RP-HPLC - MD-PH001-05020 25.00
5' Phosphate 1000 nmol RP-HPLC - MD-PH001-05100 30.00
3' Phosphate 40 nmol RP-HPLC - MD-PH001-03004 32.00
3' Phosphate 200 nmol RP-HPLC - MD-PH001-03020 40.00
3' Phosphate 1000 nmol RP-HPLC - MD-PH001-03100 60.00
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                            15-39 bases: 5 Working days
                            40-80 bases: 7-8 Working days
                            > 80 bases: 10 Working days

Packaging

   Dried

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
The most current application of the 5’-PO4 modified oligonucleotide is the direct ligation to a vector without prior use of T4 polynucleotide kinase. 3’-PO4 and 5’-PO4 oligonucleotides may also be used for either further chemical oligonucleotide modification or as a substrate in DNA repair studies. This phosphorylation permits the labelling of large quantities and is more reproducible than enzymatic procedures.





Legal notices

   For Research Use only

Leaflets

Product citations


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PEREL Y. et al., "Galanin and galanin receptor expression in neuroblastic tumours: correlation with their differentiation status", British Journal of Cancer, vol. 86, n° 1, p. 117-122, 7 January 2002


OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002


GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004


RZEM et al., "A gene encoding a putative FAD-dependent L-2-hydroxyglutarate dehydrogenase is mutated in L-2-hydroxyglutaric aciduria", PNAS, vol. 101, n° 48, 16849-16854, November 2004


SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005


VAN DER STEEGE G. et al., "Persistent failures in gene repair", Nature Biotechnology, n° 19, p. 305-306, April 2001


ISCHENKO A.A. et al., "Alternative nucleotide incision repair pathway for oxidative DNA damage", Nature, n° 415, p. 183-187, 10 January 2002


KRISTENSEN A. et al., "Detection of Mutations in Exon 8 of TP53 by Temperature Gradient 96-Capillary Array Electrophoresis", Biotechniques, n° 33, p. 650-653, September 2002


VANDEN ABEELE F. et al., "Store-operated Ca2+ Current in Prostate Cancer Epithelial Cells", Journal of Biological Chemistry, vol. 278, n° 17, p. 15381-15389, April 2003


GROS L. et al., "Hijacking of the Human Alkyl-N-purine-DNA Glycosylase by 3,N4-Ethenocytosine, a Lipid Peroxidation-induced DNA Adduct", Journal of Biological Chemistry, vol. 279, n° 17, p. 17723-17730, April 2004


MORGAN M. et al., "YY1 Regulates the Neural Crest-associated slug Gene in Xenopus laevis", Journal of Biological Chemistry, vol. 279, n° 45, p. 46826-46826, November 2004


DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004





  • Online ordering: The most convenient way to order your oligonucleotides, peptides and Real-Time qPCR kits is to use our web ordering system. To place orders online, simply login if you have already an online account.

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  • By e-mail: order@eurogentec.com
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

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