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Phosphate

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Phosphate Build & Order

The most current application of the 5’-PO4 modified oligonucleotide is the direct ligation to a vector without prior use of T4 polynucleotide kinase. 3’-PO4 and 5’-PO4 oligonucleotides may also be used for either further chemical oligonucleotide modification or as a substrate in DNA repair studies. This phosphorylation permits the labelling of large quantities and is more reproducible than enzymatic procedures.

Name Synthesis scale Recommended Purification* Required Purification* Reference EUR
5' Phosphate 40 nmol RP-HPLC - MD-PH001-05004 20.00
5' Phosphate 200 nmol RP-HPLC - MD-PH001-05020 25.00
5' Phosphate 1000 nmol RP-HPLC - MD-PH001-05100 30.00
3' Phosphate 40 nmol RP-HPLC - MD-PH001-03004 32.00
3' Phosphate 200 nmol RP-HPLC - MD-PH001-03020 40.00
3' Phosphate 1000 nmol RP-HPLC - MD-PH001-03100 60.00
* More info on the Purifications page

Quality Control

   MALDI-TOF Mass Spectrometry

Delivery times

   2-14 bases: 5 Working days
                            15-39 bases: 5 Working days
                            40-80 bases: 7-8 Working days
                            > 80 bases: 10 Working days

Packaging

   Dried

Shipping conditions

   Room temperature

Storage conditions

   -20 °C to -70 °C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
The most current application of the 5’-PO4 modified oligonucleotide is the direct ligation to a vector without prior use of T4 polynucleotide kinase. 3’-PO4 and 5’-PO4 oligonucleotides may also be used for either further chemical oligonucleotide modification or as a substrate in DNA repair studies. This phosphorylation permits the labelling of large quantities and is more reproducible than enzymatic procedures.





Legal notices

   For Research Use only

Leaflets

Product citations


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OSTERMANN G. et al., "JAM-1 is a ligand of the 2 integrin LFA-1 involved in transendothelial migration of leukocytes", Nature Immunology, vol. 3, n° 2, p.151-158, 1 February 2002


GOMEZ D. et al., "Interaction of Telomestatin with the Telomeric Single-strand Overhang", Journal of Biological Chemistry, vol. 279, n° 40, p. 41487-41494, October 2004


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SU T.-J. et al., "DNA bending by M.EcoKI methyltransferase is coupled to nucleotide flipping", Nucleic Acids Research, vol. 33, n° 10, 3235-3244, June 2005


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DI GIUSTO D. et al., "Construction, Stability, and Activity of Multivalent Circular Anticoagulant Aptamers", Journal of Biological Chemistry, vol. 279, n° 45, p. 46483-46489, November 2004





  • Online ordering: The most convenient way to order your oligonucleotides, peptides and Real-Time qPCR kits is to use our web ordering system. To place orders online, simply login if you have already an online account.

    New online customers ? Not registered yet ? Request your free login and password.

  • By e-mail: order@eurogentec.com
  • By fax : +32 4 264 07 88
  • By mail
    Eurogentec S.A.
    LIEGE Science Park
    Rue du Bois Saint Jean 5
    4102 Seraing
    BELGIUM

If you still have questions with ordering please contact our Customer Support : +32 4 372 76 65.

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